| Autor: |
El Hawari K; ANSES Fougères Laboratory, French Agency for Food, Environment and Occupational Health & Safety, French and European Union Reference Laboratory for Veterinary Medicinal Product Residues and Pharmacologically Active Dye Residues in Food, 10 B rue Claude Bourgelat - Javené, CS 40608, Fougères Cedex 35306, France., Taillandier JF; Experimentation, Modelling and Data Analysis Unit, French Agency for Food, Environmental and Occupational Health and Safety (Anses), Fougères Laboratory, Fougères Cedex 35306, France., Guichard P; ANSES Fougères Laboratory, French Agency for Food, Environment and Occupational Health & Safety, French and European Union Reference Laboratory for Veterinary Medicinal Product Residues and Pharmacologically Active Dye Residues in Food, 10 B rue Claude Bourgelat - Javené, CS 40608, Fougères Cedex 35306, France., Laurentie M; Experimentation, Modelling and Data Analysis Unit, French Agency for Food, Environmental and Occupational Health and Safety (Anses), Fougères Laboratory, Fougères Cedex 35306, France., Hurtaud-Pessel D; ANSES Fougères Laboratory, French Agency for Food, Environment and Occupational Health & Safety, French and European Union Reference Laboratory for Veterinary Medicinal Product Residues and Pharmacologically Active Dye Residues in Food, 10 B rue Claude Bourgelat - Javené, CS 40608, Fougères Cedex 35306, France., Verdon E; ANSES Fougères Laboratory, French Agency for Food, Environment and Occupational Health & Safety, French and European Union Reference Laboratory for Veterinary Medicinal Product Residues and Pharmacologically Active Dye Residues in Food, 10 B rue Claude Bourgelat - Javené, CS 40608, Fougères Cedex 35306, France. |
| Abstrakt: |
Nitrofurazone (NFZ) antibiotic is banned in food-producing animals, and its metabolite, semicarbazide (SEM), is used as a marker residue for nitrofurazone abuse. However, SEM can also be generated during food processing without veterinary treatment. Therefore, SEM cannot be considered an unequivocal marker of NFZ. This study investigates the use of 5-nitro-2-furaldehyde (5-NF) as an additional marker for nitrofurazone in broiler chickens. After oral administration of NFZ and 5-NF at 25 mg·kg -1 BW for 10 days, tissues (muscle and liver) and plasma samples were analyzed by liquid chromatography-tandem mass spectrometry. In NFZ-treated chickens, 5-NF was detected within 1 h in plasma at a peak concentration of 1.4 ± 0.25 μg·kg -1 , while in 5-NF-treated ones, the maximum concentration was 0.85 ± 0.06 μg·kg -1 after 2 h. 5-NF was not detected in muscle and liver for both treated groups from 0 to 21 days. However, SEM persisted for up to 3 weeks in muscle (0.3 ± 0.018 μg·kg -1 ), 2 weeks in liver (0.16 ± 0.008 μg·kg -1 ), and 1 week in plasma (0.4 ± 0.05 μg·kg -1 ) with a faster elimination rate in liver tissues (half-life of 4.6 days) compared to muscle tissues (half-life of 6.5 days). These findings suggest that SEM remains a better marker for detecting nitrofurazone abuse in chickens compared to 5-NF. |
