Cytotoxicity assessment of eluates from vacuum-forming thermoplastics.

Autor: Turkalj M; Department of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU Leuven, Leuven, Belgium., Ghosh M; Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium., Čokić SM; Department of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU Leuven, Leuven, Belgium., Hoet PHM; Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium.; Laboratory of Respiratory Diseases and Thoracic Surgery (BREATHE), KU Leuven, Leuven, Belgium., Vanoirbeek J; Environment and Health, Department of Public Health and Primary Care, KU Leuven, Leuven, Belgium., Van Meerbeek B; Department of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU Leuven, Leuven, Belgium., Van Landuyt KL; Department of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU Leuven, Leuven, Belgium. kirsten.vanlanduyt@uzleuven.be.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2024 Oct 04; Vol. 28 (10), pp. 570. Date of Electronic Publication: 2024 Oct 04.
DOI: 10.1007/s00784-024-05952-4
Abstrakt: Objectives: This study aimed to evaluate possible cytotoxic effects of thermoplastic materials commonly used for occlusal splints and orthodontic appliances.
Methods: Seven thermoplastics were included: three variants of the Essix sheets (C+, Plus, and Tray Rite; Dentsply Sirona), three thermoplastics (Bleach Heavy, Splint, and X-Heavy; Cavex Holland) and Invisalign (Align Technology). Cylindrical specimens (n = 24; 10 mm diameter) were incubated in cell culture medium for 24 h and 14 days. After incubation, the medium was collected, serially diluted, and dosed to primary human gingival fibroblasts in triplicate. Medium processed like the samples was used as negative control. Cell viability was evaluated by XTT and LDH assay to assess metabolic activity and membrane integrity, respectively. Next, cell cycle was assessed with flow cytometry after exposing HGFs to undiluted extracts.
Results: The 24-hour and 14-day extracts did not evoke cytotoxicity after 24-hour incubation. No significant differences in cell viability (one-way ANOVA, p > 0.05 ) in the XTT and LDH assays or in cell cycle distribution between the different materials (two-way ANOVA, p > 0.05 ).
Conclusion: The thermoplastics tested in the study showed no evident in-vitro cytotoxic effects. Further investigation should focus on determining which compounds are released from thermoplastic materials and assessing potential toxicity related to exposure to these compounds.
Clinical Significance: Our study adds to the growing body of evidence on the biocompatibility of dental thermoplastics. This can aid clinical decision-making, as thermoplastics are expected to be safe to use in terms of cytotoxicity.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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