Golden Gate Cloning for the Standardized Assembly of Gene Elements with Modular Cloning in Chlamydomonas.

Autor:	Emelin P; Molecular Plant Sciences, Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany., Abdul-Mawla S; Molecular Plant Sciences, Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany., Willmund F; Molecular Plant Sciences, Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany. willmund@staff.uni-marburg.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2850, pp. 451-465.
DOI:	10.1007/978-1-0716-4220-7_25
Abstrakt:	Modern synthetic biology requires fast and efficient cloning strategies for the assembly of new transcription units or entire pathways. Modular Cloning (MoClo) is a standardized synthetic biology workflow, which has tremendously simplified the assembly of genetic elements for transgene expression. MoClo is based on Golden Gate Assembly and allows to combine genetic elements of a library through a hierarchical syntax-driven pipeline. Here we describe the assembly of a genetic cassette for transgene expression in the single-celled model alga Chlamydomonas reinhardtii. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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