Golden Gate Cloning in Actinobacteria: Opportunities and Challenges.

Autor:	Ngo ACR; Microbial Biotechnology, Ruhr University Bochum, Bochum, Germany., Haarmann M; Microbial Biotechnology, Ruhr University Bochum, Bochum, Germany., Weindorf N; Microbial Biotechnology, Ruhr University Bochum, Bochum, Germany., Guanzon DAV; Microbial Biology, Ruhr University Bochum, Bochum, Germany., Linke V; Microbial Biotechnology, Ruhr University Bochum, Bochum, Germany., Smitka J; Microbial Biotechnology, Ruhr University Bochum, Bochum, Germany., Tischler D; Microbial Biotechnology, Ruhr University Bochum, Bochum, Germany.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2850, pp. 377-386.
DOI:	10.1007/978-1-0716-4220-7_21
Abstrakt:	As we exploit biological machineries and circuits to redesign nature, it is just important to use efficient cloning strategies and methods to heterologously express the resulting DNA constructs. Golden Gate cloning allows the assembly of multiple fragments in a single reaction, making the process efficient and seamless. Although Golden Gate strategies have already been employed for different organisms, it is still not well-established for Actinobacteria. Here, we describe methods for Golden Gate cloning and how it can be utilized for Actinobacteria. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu