Real-time visualisation of developing chick embryos cultured in transparent plastic films from the blastoderm stage until hatching.

Autor: Obara K; Takanedai Animal Clinic, 2-16-3 Narashinodai, Funabashi, Chiba, 274-0063, Japan. ka.obara@takanedai-ah.com.; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. ka.obara@takanedai-ah.com., Obara C; Takanedai Animal Clinic, 2-16-3 Narashinodai, Funabashi, Chiba, 274-0063, Japan.; Faculty of Veterinary Medicine, Imabari Campus, Okayama University of Science, 1-3 Ikoinooka, Imabari, Ehime, 794-8555, Japan., Naito M; National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan., Asano A; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan., Tajima A; Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. tajima.atsushi.gb@u.tsukuba.ac.jp.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 03; Vol. 14 (1), pp. 22790. Date of Electronic Publication: 2024 Oct 03.
DOI: 10.1038/s41598-024-72004-y
Abstrakt: Real-time visualisation of chick embryo development occurring inside an opaque eggshell is a major goal of developmental biology. This goal was partially achieved when 3-day-old embryos recovered from fertilised shelled eggs, preincubated in a conventional incubator, hatched after the egg contents had been transferred into shell-less embryo culture systems constructed of transparent plastic film placed in a plastic cup. However, the hatchable shell-less embryo culture systems described thus far cannot be used to visualise the dynamic morphological changes associated with the emergence of major organ systems during the first 3 days of embryo development, as the blastoderm area needs to be covered with an opaque membrane. Here, we present the development of a chick embryo culture system that enables omnidirectional real-time visualisation of the developing embryo, beginning from the newly laid blastoderm stage embryo until hatching.
(© 2024. The Author(s).)
Databáze: MEDLINE
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