Campylobacter species, Salmonella serotypes and ribosomal RNA-based fecal source tracking in the Kokemäki River watershed.

Autor: Hokajärvi AM; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland. Electronic address: anna-maria.hokajarvi@thl.fi., Tiwari A; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland; University of Helsinki, Department of Food Hygiene and Environmental Health, Agnes Sjöbergin katu 2, Helsinki, Finland., Räsänen P; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland., Wessels L; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland., Rankinen K; Finnish Environment Institute (Syke), Latokartanonkaari 11, Helsinki, Finland., Juntunen J; Finnish Environment Institute (Syke), Survontie 9 A, Jyväskylä, Finland., Grootens RJF; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland., Kuronen H; Finnish Food Authority, Neulaniementie 4, Kuopio, Finland., Vepsäläinen A; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland., Miettinen IT; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland; Aalto University, Water and Environmental Engineering, Tietotie 1E, Espoo, Finland., Huttula T; Finnish Environment Institute (Syke), Survontie 9 A, Jyväskylä, Finland., Pitkänen T; Finnish Institute for Health and Welfare, Neulaniementie 4, Kuopio, Finland; University of Helsinki, Department of Food Hygiene and Environmental Health, Agnes Sjöbergin katu 2, Helsinki, Finland.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Dec 01; Vol. 954, pp. 176559. Date of Electronic Publication: 2024 Oct 01.
DOI: 10.1016/j.scitotenv.2024.176559
Abstrakt: Fecal contamination of surface water compromises the usability of surface water for drinking water production due to an increase in human health risks. In this study, we collected surface water samples for two years from the Kokemäki River (Finland). The downstream river stretch is used for feeding production of artificial ground water for a major drinking water treatment plant. The prevalence of Campylobacter species and Salmonella serotypes together with fecal source identifiers targeting general, human, gull, swine, and ruminant were evaluated at 16 sampling sites throughout the studied watershed. We detected Campylobacter spp. from all 16 sampling sites with Campylobacter jejuni and Campylobacter lari as the most detected species. Salmonella spp. was detected in 10 out of 16 sampling sites, with Salmonella Typhimurium being the most common serovar. Regarding spatial variation in the hygienic quality of surface water, the upstream area (urban proximity) and downstream area (agricultural proximity) had higher microbial loads than the middle section of the study area. Samples taken in fall and spring had higher microbial loads than summer and winter samples. The lower ratio of rRNA to rRNA-gene (rDNA) of studied microbes in the winter than in other seasons may indicate low metabolic activity of bacterial targets during winter. The number of gulls, swine, and cattle in the catchment area concorded with the number of fecal source identifiers in the surface water. Further, the prevalence of gull-specific source identifier agreed with the detection of C. coli, C. lari, and S. Typhimurim, whereas the prevalence of swine- and ruminant-specific source identifiers agreed with the detection of C. jejuni and C. coli. Thus, fecal source identifiers are shown to be important tools for monitoring zoonotic pathogens affecting microbial quality of surface water. Further, variation in fecal loads indicates such variation in health risks related to surface water use.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: