Adaptations in glutathione-based redox protein signaling pathways and alcohol drinking across species.

Autor: Womersley JS; Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA; Department of Psychiatry, Stellenbosch University, Cape Town, South Africa; South African Medical Research Council / Stellenbosch University Genomics of Brain Disorders Research Unit, Department of Psychiatry, Stellenbosch University, Cape Town, South Africa., Obellianne C; Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA., Padula AE; Department of Neuroscience, Medical University of South Carolina, Charleston, SC 29425, USA., Lopez MF; Department of Psychiatry & Behavioral Sciences, Medical University of South Carolina, Charleston, SC 29425, USA., Griffin WC; Department of Psychiatry & Behavioral Sciences, Medical University of South Carolina, Charleston, SC 29425, USA., Ball LE; Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA., Berto S; Department of Neuroscience, Medical University of South Carolina, Charleston, SC 29425, USA., Grant KA; Department of Behavioral Neuroscience, Oregon Health and Science University, Portland, OR 97239, USA., Townsend DM; Department of Drug Discovery and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA., Uys JD; Department of Cell and Molecular Pharmacology & Experimental Therapeutics, Medical University of South Carolina, Charleston, SC 29425, USA., Mulholland PJ; Department of Neuroscience, Medical University of South Carolina, Charleston, SC 29425, USA. Electronic address: mulholl@musc.edu.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2024 Nov; Vol. 180, pp. 117514. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1016/j.biopha.2024.117514
Abstrakt: Alcohol use disorder (AUD) is the most prevalent substance use disorder but there is incomplete knowledge of the underlying molecular etiology. Here, we examined the cytosolic proteome from the nucleus accumbens core (NAcC) of ethanol drinking rhesus macaques to identify ethanol-sensitive signaling proteins. The targets were subsequently investigated using bioinformatics, genetic, and pharmacological manipulations in mouse models of ethanol drinking. Of the 1000+ cytosolic proteins identified in our screen, 50 proteins differed significantly between control and ethanol drinking macaques. Gene Ontology analysis of the differentially expressed proteins identified enrichment in pathways regulating metabolic processes and proteasome activity. Because the family of Glutathione S-transferases (GSTs) was enriched in these pathways, validation studies targeted GSTs using bioinformatics and genetically diverse mouse models. Gstp1 and Gstm2 were identified in Quantitative Trait Loci and published gene sets for ethanol-related phenotypes (e.g., ethanol preference, conditioned taste aversion, differential expression), and recombinant inbred strains that inherited the C57BL/6J allele at the Gstp2 interval consumed higher amounts of ethanol than those that inherited the DBA/2J allele. Genetic deletion of Gstp1/2 led to increased ethanol consumption without altering ethanol metabolism or sucrose preference. Administration of the pharmacologic activator of Gstp1/2, carnosic acid, decreased voluntary ethanol drinking. Proteomic analysis of the NAcC cytosolic of heavy drinking macaques that were validated in mouse models indicate a role for glutathione-mediated redox regulation in ethanol-related neurobiology and the potential of pharmacological interventions targeting this system to modify excessive ethanol drinking.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper As Danyelle Townsend, a co-author on this paper, is an Editor of Biomedicine and Pharmacotherapy, they were blinded to this paper during review, and the paper was independently handled by Dr. Marek Schwendt.
(Copyright © 2024. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
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