A Next-Generation Combinatorial Genomic Strategy Scans Genomic Loci Governing Heat Stress Tolerance in Chickpea.

Autor: Mohanty JK; Genomics-Assisted Breeding and Crop Improvement Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi, India., Yadav A; Genomics-Assisted Breeding and Crop Improvement Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi, India., Narnoliya L; Genomics-Assisted Breeding and Crop Improvement Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi, India., Thakro V; Genomics-Assisted Breeding and Crop Improvement Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi, India., Nayyar H; Department of Botany, Panjab University, Chandigarh, India., Dixit GP; Indian Institute of Pulses Research (IIPR), Kanpur, Uttar Pradesh, India., Jha UC; Indian Institute of Pulses Research (IIPR), Kanpur, Uttar Pradesh, India.; Sustainable Intensification Innovation Lab, Department of Agronomy, Kansas State University, Manhattan, Kansas, USA., Vara Prasad PV; Sustainable Intensification Innovation Lab, Department of Agronomy, Kansas State University, Manhattan, Kansas, USA., Agarwal P; Genomics-Assisted Breeding and Crop Improvement Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi, India., Parida SK; Genomics-Assisted Breeding and Crop Improvement Laboratory, National Institute of Plant Genome Research (NIPGR), New Delhi, India.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Oct 03. Date of Electronic Publication: 2024 Oct 03.
DOI: 10.1111/pce.15186
Abstrakt: In the wake of rising earth temperature, chickpea crop production is haunted by the productivity crisis. Chickpea, a cool season legume manifests tolerance in several agro-physiological level, which is complex quantitative in nature, and regulated by multiple genes and genetic networks. Understanding the molecular genetic basis of this tolerance and identifying key regulators can leverage chickpea breeding against heat stress. This study employed a genomics-assisted breeding strategy utilizing multi-locus GWAS to identify 10 key genomic regions linked to traits contributing to heat stress tolerance in chickpea. These loci subsequently delineated few key candidates and hub regulatory genes, such as RAD23b, CIPK25, AAE19, CK1 and WRKY40, through integrated genomics, transcriptomics and interactive analyses. The differential transcript accumulation of these identified candidates in contrasting chickpea accessions suggests their potential role in heat stress tolerance. Differential ROS accumulation along with their scavengers' transcript abundance aligning with the expression of identified candidates in the contrasting chickpea accessions persuade their regulatory significance. Additionally, their functional significance is ascertained by heterologous expression and subsequent heat stress screening. The high confidence genomic loci and the superior genes and natural alleles delineated here has great potential for swift genomic interventions to enhance heat resilience and yield stability in chickpea.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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