CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment.

Autor:	Lee J; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Goeckel ME; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Levitas A; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Colijn S; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Shin J; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO. (J.S., A.H., L.S.-K.)., Hindes A; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO. (J.S., A.H., L.S.-K.)., Mun G; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Burton Z; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Chintalapati B; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Yin Y; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Abello J; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.)., Solnica-Krezel L; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO. (J.S., A.H., L.S.-K.).; Center of Regenerative Medicine, Washington University School of Medicine, St. Louis, MO. (L.S.-K.)., Stratman A; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO. (J.L., M.E.G., A.L., S.C., G.M., Z.B., B.C., Y.Y., J.A., A.S.).
Jazyk:	angličtina
Zdroj:	Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2024 Oct 03. Date of Electronic Publication: 2024 Oct 03.
DOI:	10.1161/ATVBAHA.124.321434
Abstrakt:	Background: Endothelial cell (EC)-pericyte interactions are known to remodel in response to hemodynamic forces; yet there is a lack of mechanistic understanding of the signaling pathways that underlie these events. Here, we have identified a novel signaling network regulated by blood flow in ECs-the chemokine receptor CXCR3 (CXC motif chemokine receptor 3) and one of its ligands, CXCL11 (CXC motif chemokine ligand 11)-that delimits EC angiogenic potential and promotes pericyte recruitment to ECs during development. Methods: We investigated the role of CXCR3 on vascular development using both 2- and 3-dimensional in vitro assays, to study EC-pericyte interactions and EC behavioral responses to blood flow. Additionally, genetic mutants and pharmacological modulators were used in zebra fish in vivo to study the impacts of CXCR3 loss and gain of function on vascular development. Results: In vitro modeling of EC-pericyte interactions demonstrates that suppression of EC-specific CXCR3 signaling leads to loss of pericyte association with EC tubes. In vivo, phenotypic defects are particularly noted in the cranial vasculature, where we see a loss of pericyte association with ECs and expansion of the vasculature in zebra fish treated with the Cxcr3 inhibitor AMG487 or in homozygous cxcr3.1/3.2/3.3 triple mutants. We also demonstrate that CXCR3-deficient ECs are more elongated, move more slowly, and have impaired EC-EC junctions compared with their control counterparts. Conclusions: Our results suggest that CXCR3 signaling in ECs helps promote vascular stabilization events during development by preventing EC overgrowth and promoting pericyte recruitment.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu