Identification of proteins' expression pathway and the effective miRNAs for the treatment of human papillomavirus-induced cervical cancer: in-silico analyses-experimental research.

Autor:	Monemi M; Department of Basic Science, Faculty of Pharmacy and Pharmaceutical Science, Tehran Medical Science, Islamic Azad University, Tehran., Garrosi L; Department of Obstetrics and Gynecology, Zanjan University of Medical Sciences, Zanjan., Mirzaei S; Department of Obstetrics and Gynecology, Arash Women Hospital, Tehran University of Medical Sciences, Tehran., Farhadi B; School of Medicine, Islamic Azad University, Mashhad Branch, Mashhad., Ataee Disfani R; Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar., Zabihi MR; Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran., Akhoondian M; Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran., Ghorbani Vajargah P; Department of Medical-Surgical Nursing, School of Nursing and Midwifery, Guilan University of Medical Sciences, Rasht, Iran., Khorshid A; Department of Clinical Laboratory Sciences, University of Texas Medical Branch, Galveston, TX, USA., Karkhah S; Department of Medical-Surgical Nursing, School of Nursing and Midwifery, Guilan University of Medical Sciences, Rasht, Iran.
Jazyk:	angličtina
Zdroj:	Annals of medicine and surgery (2012) [Ann Med Surg (Lond)] 2024 Aug 30; Vol. 86 (10), pp. 5784-5792. Date of Electronic Publication: 2024 Aug 30 (Print Publication: 2024).
DOI:	10.1097/MS9.0000000000002513
Abstrakt:	Introduction: Cervical cancer is the fourth most common cancer in women. The risk factors for cervical cancer include human papillomavirus (HPV) infection, age, smoking, number of pregnancies, use of oral contraceptives, and diet. However, long-term HPV infection appears to be the main risk factor for developing cervical cancer. This in-silico analysis aims to identify the expression network of proteins and the miRNAs that play a role in the development of HPV-induced cervical cancer. Methods: The critical proteins and miRNAs were extracted using the DisGeNET and miRBase databases. String and Gephi were applied to the network analysis. The GTEx web tool was utilized to Identify tissue expression levels. The Enrichr website was used to explore the molecular function and pathways of found genes. Results: Ten proteins, TP53, MYC, AKT1, TNF, IL6, EGFR, STAT3, CTNNB1, ESR1, and JUN, were identified as the most critical shared gene network among cervical cancer and HPV. Seven miRNAs were found, including hsa-mir-146a, hsa-mir-27, hsa-mir-203, hsa-mir-126, hsa-mir-145, hsa-mir-944, and hsa-mir-93, which have a common expression in cervical cancer and HPV. Conclusion: Overall, the gene network, including TP53, MYC, AKT1, TNF, IL6, EGFR, STAT3, CTNNB1, ESR1, and JUN, and Also, hsa-mir-145, hsa-mir-93, hsa-mir-203, and hsa-mir-126 can be regarded as a gene expression pathway in HPV-induced cervical cancer. Competing Interests: The authors declare no conflict of interest. (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc.)
Databáze:	MEDLINE
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