Suramin enhances proliferation, migration, and tendon gene expression of human supraspinatus tenocytes.

Autor: Huang SH; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Wang CC; Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan., Shen PC; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.; School of Post-Baccalaureate Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.; Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan.; Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan., Liu ZM; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Chen SJ; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Tien YC; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan., Lu CC; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.; School of Post-Baccalaureate Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.; Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan.; Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan.; Department of Orthopaedic Surgery, Kaohsiung Municipal Siaogang Hospital, Kaohsiung, Taiwan.; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Jazyk: angličtina
Zdroj: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2024 Oct 02. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1002/jor.25990
Abstrakt: Rotator cuff tendinopathy is a common musculoskeletal disorder with limited pharmacological treatment strategies. This study aimed to investigate tenocytes' functional in vitro response from a ruptured supraspinatus tendon to suramin administration and to elucidate whether suramin can enhance tendon repair and modulate the inflammatory response to injury. Tenocytes were obtained from human supraspinatus tendons (n = 6). We investigated the effect of suramin on LPS-induced inflammatory responses and the underlying molecular mechanisms in THP-1 macrophages. Suramin enhanced the proliferation, cell viability, and migration of tenocytes. It also increased the protein expression of PCNA and Ki-67. Suramin-treated tenocytes exhibited increased expression of COL1A1, COL3A1, TNC, SCX, and VEGF. Suramin significantly reduced LPS-induced iNOS, COX2 synthesis, inflammatory cytokine TNF-α production, and inflammatory signaling by influencing the NF-κB pathways in THP-1 cells. Our results suggest that suramin holds great promise as a therapeutic option for treating rotator cuff tendinopathy.
(© 2024 Orthopaedic Research Society.)
Databáze: MEDLINE
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