Investigation of γ-polyglutamic acid production via asynchronous saccharification and fermentation of raw corn starch.

Autor: Gou Y; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China., Niu C; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China., Ge F; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China. 19980081@sicnu.edu.cn., Li W; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China. liwei001@sicnu.edu.cn., Cheng G; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China. chgy@sicnu.edu.cn., Jing S; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China., Yang H; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China., Li J; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China., Ren Y; Ren College of life Sciences, Sichuan Normal University, Chengdu, 610101, Sichuan, China.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2024 Oct 03; Vol. 40 (11), pp. 338. Date of Electronic Publication: 2024 Oct 03.
DOI: 10.1007/s11274-024-04141-5
Abstrakt: Starch, a crucial raw material, has been extensively investigated for biotechnological applications. However, its application in γ-polyglutamic acid (γ-PGA) production remains unexplored. Based on γ-PGA output of Bacillus subtilis SCP010-1, a novel asynchronous saccharification and fermentation process for γ-PGA synthesis was implemented. The results revealed that a starch concentration of 20%, α-amylase dosage of 75 U/g, liquefaction temperature of 72℃, and γ-PGA yield of 36.31 g/L was achieved. At a glucoamylase dosage of 100 U/g, saccharification 38 h at 60℃, the yield of γ-PGA increased to 48.88 g/L. The contents of total sugar, glucose, maltose and oligosaccharide in saccharified liquid were determined. Through batch fermentation of saccharified liquid in fermentor, the γ-PGA output was elevated to 116.08 g/L. This study can offer a potential cost reduction of 40%, which can be a promising advancement in industrial γ-PGA production. Moreover, our approach can be applied in other starch-based fermentation industries.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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