Infected wound repair correlates with collagen I induction and NOX2 activation by cold atmospheric plasma.

Autor: Blaise O; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France.; École Polytechnique, Sorbonne Université, CNRS UMR7648, Laboratoire de Physique des Plasmas, Palaiseau, France., Duchesne C; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France.; École Polytechnique, Sorbonne Université, CNRS UMR7648, Laboratoire de Physique des Plasmas, Palaiseau, France., Capuzzo E; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France., Nahori MA; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité des Toxines Bactériennes, Paris, France., Fernandes J; Institut Pasteur, UTechS PBI, C2RT, Paris, France., Connor MG; Institut Pasteur, Université Paris Cité, Unité Chromatine et Infection, Paris, France., Hamon MA; Institut Pasteur, Université Paris Cité, Unité Chromatine et Infection, Paris, France., Pizarro-Cerda J; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France., Lataillade JJ; Centre de Transfusion Sanguine des Armées, Clamart, France., McGuckin C; CTI Biotech, Meyzieu, France., Rousseau A; École Polytechnique, Sorbonne Université, CNRS UMR7648, Laboratoire de Physique des Plasmas, Palaiseau, France., Banzet S; Centre de Transfusion Sanguine des Armées, Clamart, France.; Institut de Recherche Biomédicale des Armées, INSERM UMRS-MD 1197, Brétigny-sur-Orge, France., Dussurget O; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France. olivier.dussurget@pasteur.fr., Frescaline N; Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France. nadira.frescaline@intradef.gouv.fr.; Centre de Transfusion Sanguine des Armées, Clamart, France. nadira.frescaline@intradef.gouv.fr.
Jazyk: angličtina
Zdroj: NPJ Regenerative medicine [NPJ Regen Med] 2024 Oct 02; Vol. 9 (1), pp. 28. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1038/s41536-024-00372-0
Abstrakt: Cold atmospheric plasma (CAP) is a promising complement to tissue repair and regenerative medicine approaches. CAP has therapeutic potential in infected cutaneous wounds by mechanisms which remain enigmatic. Here, CAP is shown to activate phagocyte NADPH oxidase complex NOX2. CAP induced increased intracellular reactive oxygen species, alleviated by NOX2 inhibitors. Genetic and pharmacological inhibitions of NOX2 in macrophages and bioengineered skin infected with Staphylococcus aureus and treated with CAP reduced intracellular oxidants and increased bacterial survival. CAP triggered Rac activation and phosphorylation of p40 phox and p47 phox required for NOX2 assembly and activity. Furthermore, CAP induced collagen I expression by fibroblasts. Infection and healing kinetics showed that murine skin wounds infected with S. aureus and treated with CAP are characterized by decreased bacterial burden, increased length of neoepidermis and extracellular matrix formation. Collectively, our findings identify mechanisms triggered by CAP that subdue infection and result in enhanced repair following skin injury.
(© 2024. The Author(s).)
Databáze: MEDLINE
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