Concentration-dependent microvascular responses to repeated iontophoresis of acetylcholine.

Autor: Iredahl F; Primary Health Care Center, Department of Health, Medicine and Caring Sciences, Linköping University, Linköping, Sweden; Wallenberg Centre for Molecular Medicine, Linköping University, Linköping, Sweden. Electronic address: fredrik.iredahl@liu.se., Tesselaar E; Department of Radiation Physics, Department of Medical and Health Sciences, Linköping University, Linköping, Sweden., Jonasson H; Department of Biomedical Engineering, Linköping University, Sweden., Wilhelms D; Department of Emergency Medicine in Linköping, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden., Henricson J; Department of Emergency Medicine in Linköping, Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden.
Jazyk: angličtina
Zdroj: Microvascular research [Microvasc Res] 2025 Jan; Vol. 157, pp. 104749. Date of Electronic Publication: 2024 Sep 30.
DOI: 10.1016/j.mvr.2024.104749
Abstrakt: Background: Iontophoresis studies face challenges due to the unknown absolute drug dose delivered and the possible effect of the current used in drug delivery on the microvessels, known as current-induced vasodilation. This study aimed to investigate how various concentrations of acetylcholine (ACh), delivered through transdermal iontophoresis using repeated current pulses, impact the recovery profile of the microvascular response.
Methods: The study included fifteen healthy volunteers, and microvascular responses to five concentrations of iontophorised ACh (ranging from 0.0055 mM to 55 mM) and sterile water were assessed at six forearm skin sites using polarized reflectance spectroscopy. Iontophoresis at each concentration involved three consecutive pulses separated 8 recovery periods.
Results: Current-induced responses were more pronounced for lower concentrations of ACh and for sterile water. With repeated pulses, lower concentrations of ACh exhibited a recovery profile more akin to higher concentrations.
Perspective: Through repeated iontophoresis of ACh, microvascular responses exhibit variation based on the drug concentration and the number of pulses administered. These variations are likely attributed to changes in skin conductivity and permeability.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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