BpTCP19 targets BpWRKY53 to negatively regulate jasmonic acid- and dark-induced leaf senescence in Betula platyphylla.

Autor: Wang B; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China., Kong WF; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China., Dong W; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China., Su LH; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China., Luan JY; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China., Jiang J; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China., Liu GF; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China., Li HY; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China. Electronic address: lihuiyu2017@126.com.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Nov; Vol. 216, pp. 109158. Date of Electronic Publication: 2024 Sep 27.
DOI: 10.1016/j.plaphy.2024.109158
Abstrakt: TCP (TEOSINTE-LIKE1, CYCLOIDEA, and PROLIFERATING CELL FACTOR1) is a plant-specific transcription factor that has garnered significant attention due to its wide-ranging involvement in the regulation of plant growth or developmental processes. However, the molecular mechanisms through which TCP genes orchestrate leaf senescence have not been extensively elucidated. BpTCP19, a member of the PCF subfamily in Betula platyphylla, and has high homology to AtTCP19. BpTCP19 displayed pronounced downregulation in response to methyl jasmonate (MeJA) and dark treatment. Overexpressing BpTCP19 in Betula platyphylla led to a delay in leaf senescence, resulting in prolonged leaf greenness under both MeJA and dark conditions. Transcriptome analysis revealed that overexpression of BpTCP19 induced alterations in the expression levels of genes linked to cell proliferation, hormone signaling transduction, and leaf senescence, including the early responsive factor BpWRKY53. Furthermore, through Yeast one-hybrid assays and GUS analysis, BpTCP19 was shown to bind to the promoter region of BpWRKY53, suppressing its expression and thereby retarding leaf senescence. This study elucidates the physiological and molecular functions of BpTCP19 as a central transcriptional regulatory module in leaf senescence and provides a potential target gene for delaying leaf senescence by mitigating sensitivity to external aging signals such as Jasmonic acid (JA) and darkness.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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