Epidermal distribution of tetrodotoxin-rich cells in newly hatched larvae of Takifugu spp.

Autor: Inahashi K; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan., Yonezawa R; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan., Hayashi K; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan., Watanabe S; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan., Yoshitake K; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan.; School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, 252-0373, Japan., Smith AR; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan., Kaneko Y; College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan., Watanabe I; College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan., Suo R; College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan., Kinoshita S; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan., Rafiuddin MA; Noto Center for Fisheries Science and Technology, Kanazawa University, Ossaka, Noto-Cho, Ishikawa, 927-0552, Japan., Seki Y; Noto Center for Fisheries Science and Technology, Kanazawa University, Ossaka, Noto-Cho, Ishikawa, 927-0552, Japan., Nagami A; Noto Center for Fisheries Science and Technology, Kanazawa University, Ossaka, Noto-Cho, Ishikawa, 927-0552, Japan., Matsubara H; Noto Center for Fisheries Science and Technology, Kanazawa University, Ossaka, Noto-Cho, Ishikawa, 927-0552, Japan., Suzuki N; Noto Marine Laboratory, Institute of Nature and Environmental Technology, Division of Marine Environmental Studies, Kanazawa University, Ogi, Noto-Cho, Ishikawa, 927-0553, Japan., Takatani T; Graduate School of Integrated Science and Technology, Nagasaki University, Nagasaki, 852-8521, Japan., Arakawa O; Graduate School of Integrated Science and Technology, Nagasaki University, Nagasaki, 852-8521, Japan., Suzuki M; College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan., Asakawa S; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan. asakawa@g.ecc.u-tokyo.ac.jp., Itoi S; College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan. sitoi@nihon-u.ac.jp.
Jazyk: angličtina
Zdroj: Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2024 Dec; Vol. 26 (6), pp. 1367-1374. Date of Electronic Publication: 2024 Oct 02.
DOI: 10.1007/s10126-024-10377-x
Abstrakt: Pufferfish of the genus Takifugu possess tetrodotoxin (TTX), known as "pufferfish toxin" and it is believed that pufferfish eggs and newly hatched larvae utilize TTX as a defensive substance against predators. However, the mechanism for the placement of TTX to specific cells on the larval body surface during the developmental process remains unknown. In this study, we clarify the distribution and characteristics of TTX-rich cells. We performed whole-mount immunohistochemistry (IHC) using anti-TTX monoclonal antibody on larvae of two pufferfish species, Takifugu rubripes and Takifugu alboplumbeus, just after hatching. This allowed observation of the TTX location and compared it with those of wheat germ agglutinin (WGA)-positive (periodic acid-Schiff (PAS)-positive) cells for mucous cells and IHC using anti-Na + /K + -ATPase (NKA) monoclonal antibody for ionocytes. As a result, uniformly scattered localization of TTX-rich cells was commonly observed in the epidermis of the larvae of the two Takifugu species. TTX-rich cells were WGA-negative (PAS-negative) and structurally distinct from NKA-positive cells, suggesting that TTX-rich cells are unreported small cells unique to pufferfish skin, but not mucous cells nor ionocytes.
(© 2024. The Author(s).)
Databáze: MEDLINE
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