Chronic Administration of Recombinant Human Erythropoietin Induces Angiogenesis in Healthy Mouse Brain.

Autor:	Pagonopoulou O; Neurophysiology, Democritus University of Thrace, Alexandroupolis, GRC., Papadatou V; Histology-Embryology, Democritus University of Thrace, Alexandroupolis, GRC., Tologkos S; Histology-Embryology, Democritus University of Thrace, Alexandroupolis, GRC., Efthimiadou A; Physiology, Independent Researcher, Alexandroupolis, GRC., Maria L; Histology-Embryology, Democritus University of Thrace, Alexandroupolis, GRC.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2024 Sep 01; Vol. 16 (9), pp. e68362. Date of Electronic Publication: 2024 Sep 01 (Print Publication: 2024).
DOI:	10.7759/cureus.68362
Abstrakt:	Introduction The hematopoietic growth factor erythropoietin (EPO) plays an important role in apoptosis and oxidative stress attenuation as well as the promotion of angiogenesis in several tissues. Systemically administered EPO has beneficial effects on rabbits subjected to subarachnoid hemorrhage or stroke. So far, the angiogenic effect of EPO has been documented after an experimentally induced stroke or subarachnoid hemorrhage. In our study, we examined the possible angiogenic effect of chronic treatment with recombinant human erythropoietin (rHuEPO) under normal conditions, in an attempt to clarify if the existence of a lesion or oxygen deprivation is necessary to initiate the angiogenic effect of EPO. Materials & methods BALB/c mice were used and were divided into three groups as follows: group A (no treatment), group B (saline only), and group C (7000 U rHuEPO per week for three weeks by intraperitoneal injection). The number of CD31- and CD34-positive endothelial cells was assessed in mouse brain preparations under control conditions and after treatment with rHuEPO. Results There was no difference between the mean numbers of CD31 and CD34 cells among the different groups. The mean number of vessels in group A and group B was almost the same (18 ± 2 vessels per optical field). However, the number of brain vessels in group C (EPO treatment) increased significantly by 44% compared to controls (26 ± 4 vessels per optical field, P < 0.05). Conclusion These data indicate that no lesion or oxygen deprivation is needed to initiate the angiogenic effect of EPO in healthy mouse brains. Competing Interests: Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: Animal Care and Use Committee of the Veterinary Department of Alexandroupolis Prefecture Issued protocol number PD 160/91. Animal care and handling was carried out based on Directive 86/609/EEC guidelines. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Pagonopoulou et al.)
Databáze:	MEDLINE
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