In Situ Isotope Tracing at Single-Cell Resolution Using Mass Spectrometry Imaging.

Autor:	Rietjens RGJ; Department of Internal Medicine (Nephrology), Leiden University Medical Center, Leiden, The Netherlands. r.g.j.rietjens@lumc.nl.; Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, Leiden, The Netherlands. r.g.j.rietjens@lumc.nl.; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, Leiden, The Netherlands. r.g.j.rietjens@lumc.nl., Heijs B; Bruker Daltonics GmbH & Co. KG, Bremen, Germany.; Center of Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2855, pp. 523-535.
DOI:	10.1007/978-1-0716-4116-3_28
Abstrakt:	Mass spectrometry imaging (MSI) allows for label-free spatial molecular interrogation of tissues. With advances in the field over recent years, the spatial resolution at which MSI data can be recorded has reached the single-cell level. This makes MSI complementary to other single-cell omics technologies. As metabolism is a highly dynamic process, capturing the metabolic turnover adds a valuable layer of information. Here, we describe how to set up in situ stable isotope tracing followed by MSI-enabled spatial metabolomics to perform dynamic metabolomics at the single-cell level. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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