Rapid Lipid Mediator Profiling by Convergence Chromatography-MS/MS.

Autor:	Metzler G; PharmaCadence Analytical Services, LLC, Hatfield, PA, USA., King RC; PharmaCadence Analytical Services, LLC, Hatfield, PA, USA., Fernández-Metzler C; PharmaCadence Analytical Services, LLC, Hatfield, PA, USA., Das A; Immunology and Respiratory Disease, Boehringer-Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA., Davis TG; Immunology and Respiratory Disease, Boehringer-Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA., Bajrami B; Drug Discovery Sciences, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany., Bretschneider T; Drug Discovery Sciences, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany., Kvaskoff D; Drug Discovery Sciences, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany. David.Kvaskoff@boehringer-ingelheim.com.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2855, pp. 341-354.
DOI:	10.1007/978-1-0716-4116-3_20
Abstrakt:	Bioactive lipid mediators derived from arachidonic acid constitute an attractive pool of metabolites that reflect cellular function and signaling, as well as potential biomarkers that may respond quantitatively to disease progression or pharmacological treatment. Their quantitative measurement in biological samples is complicated by the number of isomers that share common structural features, which are not easily distinguished by immunoassays or reverse phase chromatography-tandem mass spectrometry. Here, we present a method that enables the rapid analysis of a panel of over 25 biologically important eicosanoids in a 96-well format for cell culture supernatants, plasma, and organ tissues using convergence chromatography-tandem mass spectrometry to resolve these analytes of interest. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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