Rapid Analysis of Sterols in Blood-Derived Samples.

Autor:	Pebriana RB; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands.; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia., Simsek S; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands., Heijink M; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands., Giera M; Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands. m.a.giera@lumc.nl.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2855, pp. 291-302.
DOI:	10.1007/978-1-0716-4116-3_17
Abstrakt:	Dysregulations of cholesterol biosynthesis are known to be associated with several pathologies. Due to the rapid growth of clinical investigations in this research area, a specific, fast, and valid method for analyzing cholesterol, its precursors, and metabolites is required. Here, we describe a rapid method for sample preparation, separation, and quantification of sterols in blood-derived samples using polymeric solid phase extraction followed by gas chromatography-mass spectrometry. The validated method demonstrates a reliable quantification of cholesterol, its precursors, and metabolites. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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