Modular, Scalable, and Customizable LC-HRMS for Exposomics.
| Autor: | Hernandes VV; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Vienna, Austria.; Exposome Austria, Research Infrastructure and National EIRENE Node, Vienna, Austria., Warth B; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Vienna, Austria. benedikt.warth@univie.ac.at.; Exposome Austria, Research Infrastructure and National EIRENE Node, Vienna, Austria. benedikt.warth@univie.ac.at. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2855, pp. 41-66. |
| DOI: | 10.1007/978-1-0716-4116-3_3 |
| Abstrakt: | In this chapter, we describe a multi-purpose, reversed-phase liquid chromatography-high-resolution mass spectrometry (LC-HRMS) workflow for acquiring high-quality, non-targeted exposomics data utilizing data-dependent acquisition (DDA) combined with the use of toxicant inclusion lists for semi-targeted analysis. In addition, we describe expected retention times for >160 highly diverse xenobiotics in human plasma and serum samples. The method described is intended to serve as a generic LC-HRMS exposomics workflow for research and educational purposes. Moreover, it may be employed as a primer, allowing for further adaptations according to specialized research needs, e.g., by including reference and/or internal standards, by expanding to data-independent acquisition (DIA), or by modifying the list of compounds prioritized in fragmentation experiments (MS 2 ). (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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