Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily.

Autor: Schargel RD; Department of Microbiology, Cornell University, Ithaca, NY, USA. rds278@cornell.edu., Qayyum MZ; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN, USA., Tanwar AS; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN, USA., Kalathur RC; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN, USA., Kellogg EH; Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN, USA. Elizabeth.Kellogg@stjude.org.
Jazyk: angličtina
Zdroj: Nature structural & molecular biology [Nat Struct Mol Biol] 2024 Oct 01. Date of Electronic Publication: 2024 Oct 01.
DOI: 10.1038/s41594-024-01394-4
Abstrakt: RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases.
(© 2024. The Author(s).)
Databáze: MEDLINE
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