Metabolomics reveals a key role of salicylic acid in embryo abortion underlying interspecific hybridization between Hydrangea macrophylla and H. arborescens.

Autor: Feng J; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China., Chen S; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China., Chen H; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China., Dai L; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.; College of Horticulture, Nanjing Agricultural University, Nanjing, 210008, China., Qi X; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China., Ahmad MZ; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China., Gao K; Hangzhou Landscaping Incorporated, Hangzhou, 310020, China., Qiu S; Hangzhou Landscaping Incorporated, Hangzhou, 310020, China., Jin Y; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China., Deng Y; Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. dengym@jaas.ac.cn.; College of Horticulture, Nanjing Agricultural University, Nanjing, 210008, China. dengym@jaas.ac.cn.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2024 Oct 01; Vol. 43 (10), pp. 248. Date of Electronic Publication: 2024 Oct 01.
DOI: 10.1007/s00299-024-03341-5
Abstrakt: Key Message: Embryo abortion at the heart-shaped stage is the main reason for the failure of interspecific hybridization of hydrangea, and salicylic acid plays a key role during embryo abortion. Difficulties in obtaining seeds from interspecific hybridization between Hydrangea macrophylla and H. arborescens had severely restricted the process of breeding new hydrangea varieties. To clarify the cause of reproductive barriers, an interspecific hybridization was made between H. macrophylla 'Endless Summer' (female parent) and H. arborescens 'Annabelle' (male parent). The results showed that both parents' floral organs developed normally, 'Annabelle' had high pollen viability (84.83% at 8 h after incubation), and the pollen tube could enter into the ovule of 'Endless Summer' at 72 h after pollination. Therefore, the pre-fertilization barrier was not the main reason for the failure of interspecific hybridization. However, observation of the embryo development by paraffin sections showed that the embryo was aborted at the heart-shaped stage. In addition, salicylic acid (SA) content was significantly higher (fourfold, P < 0.01) at 21 days after pollination (DAP) as compared to that of 17 DAP, which means SA may be closely correlated with embryo development. A total of 957 metabolites were detected, among which 78 were significantly different. During the embryo abortion, phenylpropanoids and polyketides were significantly down-regulated, while organic oxygen compounds were significantly up-regulated. Further analysis indicated that the metabolic pathway was enriched in the shikimic acid biosynthesis pathway, which suggests that more SA was synthesized. Taken together, it can be reasonably speculated that SA plays a key role leading to embryo abortion underlying the interspecific hybridization between Hydrangea macrophylla and H. arborescens. The result is helpful to direct the breeding of hydrangea through distant hybridization.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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