Modification of gut and airway microbiota on ozone-induced airway inflammation.

Autor: Xu F; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Wu Q; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Yang L; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Sun H; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Li J; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., An Z; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Li H; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Wu H; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Song J; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China., Chen W; Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong 510080, China., Wu W; School of Public Health, Xinxiang Medical University, Xinxiang, Henan Province 453003, China. Electronic address: Wdwu2013@126.com.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Dec 01; Vol. 954, pp. 176604. Date of Electronic Publication: 2024 Sep 29.
DOI: 10.1016/j.scitotenv.2024.176604
Abstrakt: Ground-level ozone (O 3 ) has been shown to induce airway inflammation, the underlying mechanisms remain unclear. The aim of this study was to determine whether gut and airway microbiota dysbiosis, and airway metabolic alterations were associated with O 3 -induced airway inflammation. Thirty-six 8-week-old male C57BL/6 N mice were divided into 2 groups: sterile water group and broad-spectrum antibiotics group (Abx). Each group was further divided into two subgroups, filtered air group (Air) and O 3 group (O 3 ), with 9 mice in each subgroup. Mice in the Air and O 3 groups were exposed to filtered air or 1 ppm O 3 , 4 h/d for 5 consecutive days, respectively. Mice in Abx + Air and Abx + O 3 groups were exposed to filtered air or O 3 , respectively, after drinking broad-spectrum Abx. 24 h after the final O 3 exposure, mouse feces and bronchoalveolar lavage fluids (BALF) were collected and subjected to measurements of airway oxidative stress and inflammation biomarkers, 16S rRNA sequencing and metabolite profiling. Hematoxylin-eosin staining of lung tissues was applied to examine the pathological changes of lung tissue. The results showed that O 3 exposure resulted in airway oxidative stress and inflammation, as well as gut and airway microbiota dysbiosis, and airway metabolism alteration. Abx pre-treatment markedly changed gut and airway microbiota and promoted O 3 -induced metabolic disorder and airway inflammation. Spearman correlation analyses indicated that inter-related gut and airway microbiota dysbiosis and airway metabolic disorder were associated with O 3 -induced airway inflammation. Together, inhaled O 3 causes airway inflammation, which may implicate gut and airway microbiota dysbiosis and airway metabolic alterations.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The following are the supplementary data related to this article. Supplementary data to this article can be found online at https://doi.org/10.1016/j.scitotenv.2024.176604.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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