Synovial fluid does not retard fluid exudation during stress-relaxation of immature bovine cartilage.

Autor: Sise CV; Department of Biomedical Engineering, Columbia University, New York, NY, United States., Petersen CA; Department of Mechanical Engineering, Columbia University, New York, NY, United States., Yun J; Department of Biomedical Engineering, Columbia University, New York, NY, United States., Vukelic S; Department of Mechanical Engineering, Columbia University, New York, NY, United States., Hung CT; Department of Biomedical Engineering, Columbia University, New York, NY, United States., Ateshian GA; Department of Biomedical Engineering, Columbia University, New York, NY, United States; Department of Mechanical Engineering, Columbia University, New York, NY, United States. Electronic address: ateshian@columbia.edu.
Jazyk: angličtina
Zdroj: Journal of biomechanics [J Biomech] 2024 Nov; Vol. 176, pp. 112340. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1016/j.jbiomech.2024.112340
Abstrakt: Interstitial fluid load support (FLS) is a dominant mechanism of lubrication in cartilage, producing a low friction coefficient while enhancing the tissue's load bearing capabilities. Due to its viscosity, synovial fluid (SF) may retard loss of FLS by slowing the exudation of interstitial fluid from the cartilage. This study tested this hypothesis by comparing the stress-relaxation (SRL) response of immature bovine articular cartilage immersed either in phosphate buffered saline (PBS) or in healthy mature bovine SF, under unconfined compression (fluid exudation across cut lateral tissue boundary) and indentation testing (fluid exudation across articular surface). To investigate the influence of diffusion of SF molecular constituents into cartilage, the effect of incubation time in SF on SRL was also investigated. The SRL response in unconfined compression was not significantly different in PBS versus SF when compared directly (p = 0.98) and had a slope ofm = 1.00 ± 0.04 (R 2  = 0.989 ± 0.007). Samples tested in PBS exhibited characteristic relaxation times, τ PBS =42.6 ± 5.3 s andτ SF  = 40.8 ± 4.7 s, that were not significantly different (p = 0.40). Incubation time of 24 h in SF resulted in no significant difference in the SRL response (p = 0.39, m=1.03 ± 0.12; R 2 =0.983 ± 0.011, andτ PBS  = 43.4 ± 10.7 s versusτ SF  = 41.5 ± 4.8 s, p = 0.59). Indentation testing showed some statistically significant, but functionally insignificant, difference in SRL responses in PBS versus SF with a slope ofm = 0.958 ± 0.060 (R 2  = 0.957 ± 0.020, p = 0.029, andτ PBS  = 16.9 ± 2.6 s versusτ SF  = 19.4 ± 3.3 s, p = 0.073). Based on these results, we reject the hypothesis that healthy SF can retard the loss of FLS in cartilage due to its viscosity.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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