Alleviative effects of green-fabricated zinc oxide nanoparticles on acrylamide-induced oxidative and inflammatory reactions in the rat stomach via modulating gastric neuroactive substances and the MiR-27a-5p/ROS/NF-κB axis.

Autor: Abd-Elhakim YM; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Mohamed AA; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Khamis T; Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt; Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Metwally MMM; Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, King Salman International University, Ras Sidr, Egypt; Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., El-Shetry ES; Department of Anatomy, College of Medicine, University of Hail, Hail, Saudi Arabia; Department of Human Anatomy and Embryology, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt., Albaqami A; Department of Clinical Laboratory Sciences, Turabah University College, Taif University, Taif 21944, Saudi Arabia., Mawkili W; Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia., Alosaimi ME; Department of Basic Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, P.O Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: Mealosaimi@pnu.edu.sa., Alotaibi BS; Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia., ElAshmouny N; Department of Histology and cell biology, Kafrelsheikh University, Kafrelsheikh, Egypt., Dahran N; Department of Basic Medical Sciences, University of Jeddah, Jeddah, Saudi Arabia., Alsharif G; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences, P.O.Box 9515, Jeddah 21423, Saudi Arabia; Department of Biomedical Research, King Abdullah International Medical Research Center, P.O.Box 9515, Jeddah 21423, Saudi Arabia., Samak MA; Department of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt; College of medicine, University of Ha'il, Ha'il 2240, Saudi Arabia.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2024 Dec; Vol. 91, pp. 102574. Date of Electronic Publication: 2024 Sep 25.
DOI: 10.1016/j.tice.2024.102574
Abstrakt: Little is known about the effects of acrylamide (AMD) on the stomach. So, this study evaluated the effect of oral AMD exposure (20 mg/kg b.wt) on oxidative status, apoptotic, and inflammatory reactions in rat's stomach for 60 days. To explore novel targets of AMD toxicity, a more detailed molecular and immune-expression study was performed. Besides, the possible protective effect of green synthesized zinc oxide nanoparticles (G-ZNP) (10 mg/kg b.wt) was explored. The results revealed that AMD significantly provoked oxidative and lipid peroxidative damage of the stomach in terms of increased ROS and MDA but reduced SOD, CAT, GSH, and GSH/GSSG. Additionally, the stomachs of AMD-exposed rats showed a significant increment of PGE2 but reduced NO. Histopathologically, AMD induced a significant increase in PAS stain and the immunoexpression of iNOS and NF-κB in the glandular stomach. A significant upregulation of CART, VACHT, EGFR, caspase-3, NOS-1, and miR-27a-5p was evident in the stomach of the AMD group. Yet, G-ZNP oral dosing significantly rescued the AMD-induced oxidative damage, apoptotic reaction, inflammatory effect, and altered miR-27a-5p and gene expressions in the stomach. Conclusively, these findings demonstrated the efficacy of G-ZNP in protecting against the harmful impacts of acrylamide on stomach tissues.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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