IL-37 Inhibits Inflammation of Lacrimal Gland in Dry Eye Mice via the IL-37-PTEN-NFκB Signaling Pathway.

Autor:	Qi X; Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China., Yang Y; Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China., Xiong D; Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China., Lin B; Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China., Wu S; Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China., Chen M; The First School of Clinical Medicine, Anhui Medical University, Hefei, Anhui, China., Jiang Z; Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China., Zhang Q; Department of Ophthalmology, The Second Hospital of Anhui Medical University, Hefei, Anhui, China.
Jazyk:	angličtina
Zdroj:	Ocular immunology and inflammation [Ocul Immunol Inflamm] 2024 Dec; Vol. 32 (10), pp. 2449-2458. Date of Electronic Publication: 2024 Oct 01.
DOI:	10.1080/09273948.2024.2409371
Abstrakt:	Purpose: This study aims to investigate the role of Interleukin-37 (IL-37) in mouse models of dry eye. Methods: Two murine models of dry eye were employed in this investigation.   The evaluation of the anti-inflammatory impact of IL-37 (200 μl, 10 μg/ml) on dry eye mice involved intraperitoneal injections administered once daily for 7 days. Additionally, intraperitoneal injection of VO-Ohpic trihydrate (VO, 0.25 mg/kg) in dry eye mice was performed to investigate the role of PTEN in the IL-37 anti-inflammatory signaling pathway. Tear production was assessed using phenol red cotton thread, while corneal damage was examined through sodium fluorescein staining using a slit lamp. Histological alterations in the lacrimal gland were observed through H&E staining. PAS staining was used to assess conjunctival goblet cells. The levels of NFκB-P65, p-NFκB-P65, IL-1β, IL-6, TNF-α, CD3, AQP5, α-SMA and PTEN proteins were determined via Western blotting or immunofluorescence. Results: Following IL-37 treatment, both dry eye models exhibited reduced corneal fluorescence staining scores and enhanced tear production. In lacrimal gland, the expression of p-NFκB-P65, IL-1β, IL-6, CD3 and TNF-α was diminished, while PTEN, AQP5, α-SMA expression increased after IL-37 treatment in both dry eye mice. However, the intraperitoneal injection of VO significantly attenuated the anti-inflammatory effect of IL-37 on dry eye mice. Conclusion: IL-37 emerges as an anti-inflammatory mediator within the lacrimal gland of dry eye mice, exerting its effects through the IL-37-PTEN-NFκB signaling pathway.
Databáze:	MEDLINE
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