CRISPR/Cas-Mediated Gene Editing in Plant Immunity and Its Potential for the Future Development of Fungal, Oomycete, and Bacterial Pathogen-Resistant Pulse Crops.

Autor: Singer SD; Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada., Mukthar MM; Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada., Subedi U; Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada., Poudel H; Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada., Chen G; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada., Foroud N; Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada., Chatterton S; Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Oct 01. Date of Electronic Publication: 2024 Oct 01.
DOI: 10.1111/pce.15174
Abstrakt: Pulses provide myriad health benefits and are advantageous in an environmental context as a result of their leguminous nature. However, phytopathogenic fungi, oomycetes and bacteria pose a substantial threat to pulse production, at times leading to crop failure. Unfortunately, existing disease management strategies often provide insufficient control, and there is a clear need for the development of new pulse cultivars with durable and broad-spectrum disease resistance. CRISPR/Cas-mediated gene editing has proven its potential for rapidly enhancing disease resistance in many plant species. However, this tool has only very recently been applied in pulse species, and never in the context of plant immunity. In this review, we examine the recent successful utilization of this technology in pulse species for proof-of-concept or the improvement of other traits. In addition, we consider various genes that have been edited in other plant species to reduce susceptibility to pathogens, and discuss current knowledge regarding their roles in pulses. Given the functional conservation of the selected genes across diverse plant species, there is a high likelihood that their editing would elicit similar effects in non-oilseed grain legumes, thus providing a suite of potential targets for CRISPR/Cas-mediated gene editing to promote pulse crop productivity in coming years.
(© 2024 His Majesty the King in Right of Canada and The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd. Reproduced with the permission of the Minister of Agriculture and Agri‐Food Canada.)
Databáze: MEDLINE
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