The antimicrobial peptide Microcin C7 inhibits the growth of Porphyromonas gingivalis and improves the perodontal status in a rat model.

Autor:	Liu N; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710000, China., Zhang Q; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Jiaotong University, Xi'an 710000, China., Li J; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710000, China., Zhou S; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710000, China., Miao D; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710000, China., Zhang S; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710000, China., Chen Y; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Jiaotong University, Xi'an 710000, China.; Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an 710000, China.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2024 Nov 04; Vol. 135 (11).
DOI:	10.1093/jambio/lxae247
Abstrakt:	Aims: This study aimed to investigate the antibacterial and anti-inflammatory effects of the antimicrobial peptide Microcin C7 for Porphyromonas gingivalis-associated diseases. Methods and Results: Reverse-phase high-performance liquid chromatography revealed that Microcin C7 could remain 25.5% at 12 h in saliva. At a concentration of <10 mg ml-1, Microcin C7 showed better cytocompatibility, as revealed by a hemolysis test and a subchronic systemic toxicity test. Moreover, the minimum inhibitory concentration and minimum bactericidal concentration of Microcin C7 were analyzed using a broth microdilution method, bacterial growth curve, scanning electron microscopy, and confocal laser microscopy and determined to be 0.16 and 5 mg ml-1, respectively. Finally, in a rat model, 5 mg ml-1 Microcin C7 showed better performance in decreasing the expression of inflammatory factors (IL-1β, IL-6, IL-8, and TNF-α) and alveolar bone resorption than other concentrations. Conclusions: Microcin C7 demonstrated favorable biocompatibility, antibacterial activity, and anti-inflammatory effect, and could decrease the alveolar bone resorption in a rat model, indicating the promising potential for clinical translation and application on P. gingivalis-associated diseases. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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