Incomplete filling in the basal region of maize endosperm: timing of development of starch synthesis and cell vitality.

Autor: Chen XM; State Key Laboratory of Maize Bio-breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China., Wang ZW; State Key Laboratory of Maize Bio-breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China., Liang XG; Ministry of Education Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Agronomy College, Jiangxi Agricultural University, Jiangxi, 330045, China., Li FY; State Key Laboratory of Maize Bio-breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China., Li BB; State Key Laboratory of Maize Bio-breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China., Wu G; Agronomy College, Anhui Agricultural University, Hefei, 230036, China., Yi F; State Key Laboratory of Maize Bio-breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China., Setter TL; Section of Soil and Crop Sciences, School of Integrative Plant Science, Cornell University, Ithaca, New York, 14853, USA., Shen S; State Key Laboratory of Maize Bio-breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.; Innovation Center of Agricultural Technology for Lowland Plain of Hebei, Wuqiao, 061802, China., Zhou SL; State Key Laboratory of Maize Bio-breeding, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.; Innovation Center of Agricultural Technology for Lowland Plain of Hebei, Wuqiao, 061802, China.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Nov; Vol. 120 (3), pp. 1142-1158. Date of Electronic Publication: 2024 Sep 30.
DOI: 10.1111/tpj.17043
Abstrakt: Starch synthesis in maize endosperm adheres to the basipetal sequence from the apex downwards. However, the mechanism underlying nonuniformity among regions of the endosperm in starch accumulation and its significance is poorly understood. Here, we examined the spatiotemporal transcriptomes and starch accumulation dynamics in apical (AE), middle (ME), and basal (BE) regions of endosperm throughout the filling stage. Results demonstrated that the BE had lower levels of gene transcripts and enzymes facilitating starch synthesis, corresponding to incomplete starch storage at maturity, compared with AE and ME. Contrarily, the BE showed abundant gene expression for genetic processing and slow progress in physiological development (quantified by an index calculated from the expression values of development progress marker genes), revealing a sustained cell vitality of the BE. Further analysis demonstrated a significant parabolic correlation between starch synthesis and physiological development. An in-depth examination showed that the BE had more active signaling pathways of IAA and ABA than the AE throughout the filling stage, while ethylene showed the opposite pattern. Besides, SNF1-related protein kinase1 (SnRK1) activity, a regulator for starch synthesis modulated by trehalose-6-phosphate (T6P) signaling, was kept at a lower level in the BE than the AE and ME, corresponding to the distinct gene expression in the T6P pathway in starch synthesis regulation. Collectively, the findings support an improved understanding of the timing of starch synthesis and cell vitality in regions of the endosperm during development, and potential regulation from hormone signaling and T6P/SnRK1 signaling.
(© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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