Effects of Beraprost on Intestinal Microcirculation and Barrier Function in a Mouse Model of Renal Failure.

Autor: Hirano A; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Kadoya H; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan.; Department of General Geriatric Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan., Takasu M; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Iwakura T; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Kajimoto E; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Tatsugawa R; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Matsuura T; Toray Industries, Inc., Tokyo, Japan., Kurumatani H; Toray Industries, Inc., Tokyo, Japan., Yamamoto T; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan.; Department of General Geriatric Medicine, Kawasaki Medical School, Kurashiki, Okayama, Japan., Kidokoro K; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Kishi S; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Nagasu H; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Sasaki T; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan., Kashihara N; Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama, Japan.; Department of Medical Science, Kawasaki Medical School, Kurashiki, Okayama, Japan.
Jazyk: angličtina
Zdroj: Microcirculation (New York, N.Y. : 1994) [Microcirculation] 2024 Nov; Vol. 31 (8), pp. e12889. Date of Electronic Publication: 2024 Sep 30.
DOI: 10.1111/micc.12889
Abstrakt: Objective: Endothelial dysfunction plays an important role in the pathogenesis of chronic kidney disease. Prostacyclin (PGI 2 ), an endothelial cell-produced endogenous prostaglandin, plays a crucial role in maintaining endothelial function. However, its effects on intestinal microcirculation and barrier function are not fully understood. We hypothesized that PGI 2 improves intestinal microcirculation and barrier function via endothelial protective effects.
Methods: ICR and ICGN (a spontaneous nephrotic model) mice were used in this study. Intestinal microcirculation was visualized in vivo to investigate PGI 2 effects. Beraprost served as PGI 2 . PGI 2 administration spanned 4 weeks, following which we assessed its influence on intestinal endothelial, intestinal barrier, and renal functions.
Results: We visualized intestinal microcirculation and endothelial glycocalyx in the intestinal blood vessels. Beraprost administration induced a 1.2-fold dilatation of the vascular diameter of the small intestine. Intestinal blood flow in ICGN mice was significantly reduced compared that in ICR mice but improved with beraprost administration. ICGN mice exhibited reduced serum albumin levels, decreased ambulation, an imbalance in intestinal reactive oxygen species (ROS)/nitric oxide (NO), and impaired tight junctions; all were ameliorated by beraprost administration.
Conclusions: Beraprost improves intestinal microcirculation and barrier function by ameliorating ROS/NO imbalances, thereby reducing physical inactivity during renal failure.
(© 2024 The Author(s). Microcirculation published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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