The genomic diversity and antimicrobial resistance of Non-typhoidal Salmonella in humans and food animals in Northern India.

Autor:	Mahindroo J; Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.; MRC Centre for Global Infectious Disease Analysis, School of Public Health, Imperial College London, London, UK., Thanh DP; Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Programme, Ho Chi Minh City, Viet Nam., Kaur H; Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India., Nguyen THT; Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Programme, Ho Chi Minh City, Viet Nam., Carey ME; Department of Infection Biology, London School of Hygiene & Tropical Medicine, London, UK.; IAVI, Chelsea & Westminster Hospital, London, UK., Verma R; Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India., Mohan B; Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India., Thakur S; College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA., Baker S; Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID), Department of Medicine, University of Cambridge, Cambridge, United Kingdom.; IAVI, Chelsea & Westminster Hospital, London, UK., Taneja N; Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Jazyk:	angličtina
Zdroj:	One health (Amsterdam, Netherlands) [One Health] 2024 Sep 12; Vol. 19, pp. 100892. Date of Electronic Publication: 2024 Sep 12 (Print Publication: 2024).
DOI:	10.1016/j.onehlt.2024.100892
Abstrakt:	Introduction: Non-typhoidal Salmonella (NTS) serovars are the leading global cause of gastroenteritis and have established reservoirs in food animals. Gap Statement: Due to a lack of surveillance, there is limited information on the distribution of NTS serovars in India. Aim: Here, we investigated the epidemiology, sequence types, serovar distribution, phylogenetic relatedness, and antimicrobial resistance patterns of NTS in humans and animals across a large geographic area in Northern India. Methodology: We collected stool samples from patients with diarrhea who presented to 14 laboratories in Chandigarh and from five states in India (Punjab, Haryana, Uttarakhand, Himachal Pradesh, and Rajasthan). We sequenced the genomes and analyzed 117 NTS organisms isolated from humans and animals. Minimum inhibitory concentrations (MICs) were estimated using a Vitek2 system. Results: The prevalence of NTS in participants presenting to our study with diarrhea was 1.28 %, affecting all age groups. All NTS caused moderate to severe diarrhea. We found a high diversity of serovars with considerable serovar and sequence types (STs) overlap and phylogenetic closeness between isolates from human infections and food animals. We report serovars such as S. Agona, S. Bareilly, S. Kentucky, S. Saintpaul, and S. Virchow, causing human infections from north India for the first time. Among the different food-producing animals, pigs appeared to be a key source of human infections. Twenty-eight percent (28 %) of the NTS isolates were multi-drug resistant (MDR), and human isolates showed a higher proportion of resistance. A higher level of contamination of meat samples in our study (8.4 %) potentially suggests a close association of NTS serovars with the food chain and high transmission risk in north India. Conclusions: This study provides information on AMR genes and plasmid replicons associated with different serovars and highlights the role of food animals in AMR dissemination in our region. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
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