The Drosophila maternal-effect gene abnormal oocyte ( ao ) does not repress histone gene expression.
| Autor: | Takenaka R; Molecular and Cellular Biology Graduate Program, University of Washington, Seattle WA.; Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle WA 98109., Simmerman SM; Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle WA 98109., Schmidt CA; Department of Biology, Emory University, Atlanta GA 30322.; Biology Department, Lafayette College, Easton PA 18042., Albanese EH; Department of Biology, Emory University, Atlanta GA 30322., Rieder LE; Department of Biology, Emory University, Atlanta GA 30322., Malik HS; Division of Basic Sciences, Fred Hutchinson Cancer Center, Seattle WA 98109.; Howard Hughes Medical Institute, Fred Hutchinson Cancer Center, Seattle WA 98109. |
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| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 18. Date of Electronic Publication: 2024 Sep 18. |
| DOI: | 10.1101/2024.09.17.613536 |
| Abstrakt: | The abnormal oocyte ( ao ) gene of Drosophila melanogaster is a maternal-effect lethal gene previously identified as encoding a transcriptional regulator of core histones. However, background genetic mutations in existing ao mutant strains could compromise their utility in manipulating histone levels. To distinguish the true ao phenotype from background effects, we created two new ao reagents: a CRISPR/Cas9-mediated knockout of the ao allele for genetic and molecular analyses and an epitope-tagged ao allele for cytological experiments. Using these reagents, we confirm previous findings that ao exhibits maternal-effect lethality, which can be rescued by either a decrease in the histone gene copy number or by Y chromosome heterochromatin. We also confirm that the Ao protein localizes to the histone locus bodies in ovaries. Our data also suggest that ao genetically interacts with the histone genes and heterochromatin, as previously suggested. However, contrary to prior findings, we find that ao does not repress core histone transcript levels. Thus, the molecular basis for ao -associated maternal-effect lethality remains unknown. Competing Interests: Competing interest statement The authors declare they have no competing interests. |
| Databáze: | MEDLINE |
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