Strand dependent bypass of DNA lesions during fork reversal by ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF.

Autor:	Adolph MB; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.; Present Address: Department of Biochemistry and Molecular Biology, Saint Louis School of Medicine, St. Louis MO 63104., Warren GM; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232.; Present Address: Molecular Biology Program, Sloan Kettering Institute, New York NY 10065., Couch FB; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.; Present Address: Twin Sun LLC, Nashville TN 37203., Greer BH; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232., Eichman BF; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.; Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232., Cortez D; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Sep 19. Date of Electronic Publication: 2024 Sep 19.
DOI:	10.1101/2024.09.17.613558
Abstrakt:	During DNA replication, the replisome encounters obstacles including DNA lesions, transcription-replication conflicts, and other sources of replication stress. These obstacles must be efficiently overcome to complete DNA synthesis and minimize genome instability. One pathway to tolerate replication stress is replication fork reversal, in which parental template DNA strands are reannealed and a nascent-nascent DNA duplex is formed. Several enzymes promote replication fork reversal, including the ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF. How these enzymes translocate on DNA that contains fork-stalling lesions is unknown. Here, we examined the abilities of SMARCAL1, ZRANB3, and HLTF to tolerate various lesions on leading or lagging template strands. We demonstrate that SMARCAL1 and ZRANB3 are selectively inhibited by lesions on the leading template strand, whereas HLTF is insensitive to bulky lesions on either strand. These results suggest that SMARCAL1 and ZRANB3 contact the leading strand during fork reversal and therefore are more sensitive to inhibition by bulky lesions on this strand. In contrast, HLTF DNA translocation is inherently insensitive to DNA lesions. These biochemical differences between the fork reversal enzymes provide insights into their mechanism of DNA remodeling and suggest they may act in lesion-specific contexts. Competing Interests: Conflict of Interest The authors declare that they have no conflicts of interest with the contents of this article.
Databáze:	MEDLINE
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