| Autor: |
Chen L; Howard Hughes Medical Institute, The University of Texas at Austin, Austin, TX, 78712 USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, 78712 USA.; Department of Biology, University of Washington, Seattle, WA, 98195 USA., Maes M; Howard Hughes Medical Institute, The University of Texas at Austin, Austin, TX, 78712 USA.; Department of Biology, University of Washington, Seattle, WA, 98195 USA., Cochran AM; Howard Hughes Medical Institute, The University of Texas at Austin, Austin, TX, 78712 USA., Avila JR; Howard Hughes Medical Institute, The University of Texas at Austin, Austin, TX, 78712 USA.; Department of Biology, University of Washington, Seattle, WA, 98195 USA., Derbyshire P; The Sainsbury Laboratory, Colney Lane, NR4 7UH, Norwich, UK., Sklenar J; The Sainsbury Laboratory, Colney Lane, NR4 7UH, Norwich, UK., Haas KM; Department of Genome Sciences, University of Washington, Seattle, WA, 98195 USA., Villén J; Department of Genome Sciences, University of Washington, Seattle, WA, 98195 USA., Menke FLH; The Sainsbury Laboratory, Colney Lane, NR4 7UH, Norwich, UK., Torii KU; Howard Hughes Medical Institute, The University of Texas at Austin, Austin, TX, 78712 USA.; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, 78712 USA.; Department of Biology, University of Washington, Seattle, WA, 98195 USA. |
| Abstrakt: |
Dynamic control of signaling events requires swift regulation of receptors at an active state. By focusing on Arabidopsis ERECTA (ER) receptor kinase, which perceives peptide ligands to control multiple developmental processes, we report a mechanism preventing inappropriate receptor activity. The ER C-terminal tail (ER_CT) functions as an autoinhibitory domain: its removal confers higher kinase activity and hyperactivity during inflorescence and stomatal development. ER_CT is required for the binding of a receptor kinase inhibitor, BKI1, and two U-box E3 ligases PUB30 and PUB31 that inactivate activated ER. We further identify ER_CT as a phosphodomain transphosphorylated by the co-receptor BAK1. The phosphorylation impacts the tail structure, likely releasing from autoinhibition. The phosphonull version enhances BKI1 association, whereas the phosphomimetic version promotes PUB30/31 association. Thus, ER_CT acts as an off-on-off toggle switch, facilitating the release of BKI1 inhibition, enabling signal activation, and swiftly turning over the receptors afterwards. Our results elucidate a mechanism fine-tuning receptor signaling via a phosphoswitch module, keeping the receptor at a low basal state and ensuring the robust yet transient activation upon ligand perception. |
