TasA Fibre Interactions Are Necessary for Bacillus subtilis Biofilm Structure.

Autor: Bamford NC; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Morris RJ; National Biofilms Innovation Centre, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK., Prescott A; Centre for Advanced Scientific Technologies, School of Life Sciences, University of Dundee, Dundee, UK., Murphy P; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Erskine E; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., MacPhee CE; National Biofilms Innovation Centre, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK., Stanley-Wall NR; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2024 Oct; Vol. 122 (4), pp. 598-609. Date of Electronic Publication: 2024 Sep 30.
DOI: 10.1111/mmi.15315
Abstrakt: The extracellular matrix of biofilms provides crucial structural support to the community and protection from environmental perturbations. TasA, a key Bacillus subtilis biofilm matrix protein, forms both amyloid and non-amyloid fibrils. Non-amyloid TasA fibrils are formed via a strand-exchange mechanism, whereas the amyloid-like form involves non-specific self-assembly. We performed mutagenesis of the N-terminus to assess the role of non-amyloid fibrils in biofilm development. We find that the N-terminal tail is essential for the formation of structured biofilms, providing evidence that the strand-exchange fibrils are the active form in the biofilm matrix. Furthermore, we demonstrate that fibre formation alone is not sufficient to give structure to the biofilm. We build an interactome of TasA with other extracellular protein components, and identify important interaction sites. Our results provide insight into how protein-matrix interactions modulate biofilm development.
(© 2024 The Author(s). Molecular Microbiology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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