Favourable HDL composition in endurance athletes is not associated with changes in HDL in vitro antioxidant and endothelial anti-inflammatory function.

Autor:	Beazer JD; School of Cardiovascular and Metabolic Health, Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, United Kingdom., Sillars A; School of Cardiovascular and Metabolic Health, Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, United Kingdom., Beck S; School of Cardiovascular and Metabolic Health, Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, United Kingdom., Christoffersen C; Department of Clinical Biochemistry, Section 3-01-3, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark and Institute of Biomedical Sciences, University of Copenhagen, Blegdamsvej 3A, 2200 Copenhagen, Denmark., Ferraz MJ; Medical Biochemistry, Leiden Institute of Chemistry, Leiden University, Gorlaeus Building, Einsteinweg 55, 2333 CC Leiden, The Netherlands., Mulder MT; Division of Pharmacology, Vascular and Metabolic Diseases, Department of Internal Medicine, Erasmus University Medical Centre, Dr Molewaterplein 40, 3015 GD Rotterdam, The Netherlands., Graham D; School of Cardiovascular and Metabolic Health, Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, United Kingdom., Karlsson H; Occupational and Environmental Medicine Center in Linköping, Department of Health, Medicine and Caring Sciences, Linköping University, SE-58183 Linköping, Sweden., Ljunggren S; Occupational and Environmental Medicine Center in Linköping, Department of Health, Medicine and Caring Sciences, Linköping University, SE-58183 Linköping, Sweden., Gill J; School of Cardiovascular and Metabolic Health, Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, United Kingdom., Freeman DJ; School of Cardiovascular and Metabolic Health, Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, United Kingdom.
Jazyk:	angličtina
Zdroj:	Bioscience reports [Biosci Rep] 2024 Oct 30; Vol. 44 (10).
DOI:	10.1042/BSR20241165
Abstrakt:	Given the failure of high-density lipoprotein (HDL) raising therapies to reduce cardiovascular disease risk, attention has turned towards HDL composition and vascular protective functions. In individuals with insulin resistance, exercise interventions recover HDL function. However, the effect of exercise on HDL in otherwise healthy individuals is unknown. This cross-sectional study aimed to measure HDL composition and antioxidant/endothelial anti-inflammatory function in insulin sensitive endurance athlete and healthy control men. HDL was isolated using density gradient ultracentrifugation. HDL composition was measured using microplate assays for apolipoprotein A-I, total cholesterol content and apolipoprotein M. HDL protein composition was measured using nano-liquid chromatography tandem mass spectrometry. HDL subclass distribution was measured by native gel electrophoresis. HDL in vitro antioxidant function was measured by paraoxonase-1 activity assay and anti-inflammatory function assessed in endothelial cells. Compared with controls, endurance athlete HDL had higher apolipoprotein A-1 (1.65 ± 0.62 mg/ml vs 1.21 ± 0.34 mg/ml, P=0.028) and higher total cholesterol content (2.09 ± 0.44 mmol/L vs 1.54 ± 0.33 mmol/L, P<0.001). Proteomics revealed higher apolipoprotein A-II, A-IV and D and transthyretin in endurance athlete HDL versus controls. There was no difference observed in in vitro HDL antioxidant or anti-inflammatory functions between controls and endurance athletes. Despite a more favourable composition, endurance athlete HDL did not have higher in vitro antioxidant or anti-inflammatory function. It is possible that HDL has a ceiling of function, i.e. that healthy HDL function cannot be enhanced by endurance exercise. (© 2024 The Author(s).)
Databáze:	MEDLINE
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