The Possible Role of Progesterone Receptors in Prostate Cancer Incidences in the Iraqi Population.

Autor:	Asaad JI; Department of Medical Laboratory Techniques, Uruk University, 10068 Baghdad, Iraq., Alazzawi KSA; Department of Environmental Biotechnology, Al-Nahrain University, Biotechnology Research Center, 64074 Baghdad, Iraq., Rasheed SS; Department of Environmental Biotechnology, Al-Nahrain University, Biotechnology Research Center, 64074 Baghdad, Iraq., Algafari RN; Department of Environmental Biotechnology, Al-Nahrain University, Biotechnology Research Center, 64074 Baghdad, Iraq., Ramadhan RS; Department of Medical Laboratory Techniques, Al-Esraa University, 11101 Baghdad, Iraq., Qamandar MA; National Center for Hematology Research and Treatment, Al-Mustansiriya University, 10053 Baghdad, Iraq., Talib SS; Department of Environmental Biotechnology, Al-Nahrain University, Biotechnology Research Center, 64074 Baghdad, Iraq., Fadhil RZ; Department of Environmental Biotechnology, Al-Nahrain University, Biotechnology Research Center, 64074 Baghdad, Iraq.
Jazyk:	angličtina
Zdroj:	Frontiers in bioscience (Scholar edition) [Front Biosci (Schol Ed)] 2024 Sep 24; Vol. 16 (3), pp. 16.
DOI:	10.31083/j.fbs1603016
Abstrakt:	Background: Prostate cancer (PCa) is one of the leading diseases causing mortality. It comes in the third rank of common cancer types. It is considered extremely a complicated cancer type since it occurs in highly steroid-responsive and dependent tissues. Many factors are considered to play an important role in the disease progression of PCa, with some functioning at the molecular level. Methodology: After applying the exclusion criteria, 200 patients who underwent proctectomy were included in this study. Following receiving patient consent, blood samples were withdrawn from patients, DNA was extracted, and precise polymerase chain reaction (PCR) amplification was conducted using specifically designed primers. The resulting amplicons were sequenced and analyzed. Results: The progesterone receptor B ( PGRB ) DNA from patients showed four distinctive single-nucleotide polymorphisms (SNPs) at sites 11:101128812, 11:101128924, 11:101128949, and 11:101128986, which altered the amino acid sequences to Y>N, A>D, T>I, and C>R, respectively, compared to control. These SNPs resided in sensitive sites that either affected the control elements or promoted alterations in the protein configuration. This DNA change diminished the PGR gene function and promoted an imbalance in the encoded PGR protein structure and expression. Conclusions: Many factors may play a role in PCa manifestation, with steroids and progesterone initially noted as factors. Many studies have dealt with the hormonal effect on PCa; however, few have ultimately determined the molecular impact on disease progression. The presence of pathogenic SNPs in the enhancing region of the gene may impact the expression level of PGR . High or low expression levels may negatively affect gene function, which can be considered a reliable factor in prostate tumorigenesis. (© 2024 The Author(s). Published by IMR Press.)
Databáze:	MEDLINE
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