Discovery of a conserved translationally repressive upstream open reading frame within the iron-deficiency response regulator IDEF2.

Autor: Carey-Fung O; School of BioSciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Beasley JT; School of BioSciences, The University of Melbourne, Parkville, VIC, 3010, Australia., Broad RC; Department of Animal, Plant and Soil Sciences, School of Agriculture, Biomedicine and Environment, La Trobe University, Bundoora, VIC, 3086, Australia., Hellens RP; GenXtraits, Alameda, CA, USA., Johnson AAT; School of BioSciences, The University of Melbourne, Parkville, VIC, 3010, Australia. johnsa@unimelb.edu.au.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 Sep 30; Vol. 24 (1), pp. 891. Date of Electronic Publication: 2024 Sep 30.
DOI: 10.1186/s12870-024-05473-y
Abstrakt: Background: Iron (Fe) deficiency affects 30-50% of the world's population. Genetic biofortification of staple crops is a promising strategy for improving human nutrition, but the number of effective precision breeding targets for Fe biofortification is small. Upstream open reading frames (uORFs) are cis-regulatory elements within the 5' leader sequence (LS) of genes that generally repress translation of the main open reading frame (mORF).
Results: We aligned publicly available rice (Oryza sativa L.) ribo-seq datasets and transcriptomes to identify putative uORFs within important Fe homeostasis genes. A dual luciferase assay (DLA) was used to determine whether these uORFs cause repression of mORF translation and pinpoint LS regions that can be mutated for mORF derepression. A translationally repressive uORF region was identified in two positive regulators of the Fe-deficiency response: IDEF1 and IDEF2. The IDEF2-uORF peptide was highly conserved among monocots and a mutation series in the 5' LS of the wheat (Triticum aestivum L.) TaIDEF2-A1 gene demonstrated variable mORF derepression.
Conclusions: Together these results reveal a possible regulatory mechanism by which IDEF2 transcription factors modulate the Fe deficiency response in monocots, and highlight novel precision breeding targets to improve crop nutrition and abiotic stress tolerance.
(© 2024. The Author(s).)
Databáze: MEDLINE
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