| Autor: |
Saito K; Department of Bacteriology, Toyama Institute of Health, Japan., Kimata K; Department of Bacteriology, Toyama Institute of Health, Japan., Watahiki M; Department of Bacteriology, Toyama Institute of Health, Japan., Isobe J; Department of Bacteriology, Toyama Institute of Health, Japan., Kanatani JI; Department of Bacteriology, Toyama Institute of Health, Japan., Ikeda K; Department of Bacteriology, Toyama Institute of Health, Japan., Takeuchi T; Environmental Health division in Toyama Prefecture, Japan., Matsuzaki C; Toyama Prefectural Takaoka Health and Welfare Center, Imizu branch, Japan., Oishi K; Department of Bacteriology, Toyama Institute of Health, Japan. |
| Abstrakt: |
Clostridium perfringens, which produces C. perfringens enterotoxin (CPE), is a major causative agent of food poisoning owing to its gastrointestinal symptoms. Genotyping is important for identifying the etiological agent in outbreaks of C. perfringens. We attempted to genotype strains isolated from an outbreak of food poisoning in Toyama in 2023 using single nucleotide polymorphism (SNP) analysis. The strains of C. perfringens were isolated from a piece of curry food consumed by all patients and from the feces of the patients and employees. The cpe gene was detected in isolates from patients and curry food. The cpe-negative isolates were found in patients who consumed curry foods and in employees. The results of the SNP analysis suggest that the patient- and curry-derived isolates were likely from the same source but were unlikely to be related to the employee-derived isolates. The results of the SNP and pulsed-field gel electrophoresis (PFGE) analyses were consistent, indicating that the patient- and curry-derived isolates came from the same source. SNP analysis, a whole-genome-based genotyping method, is a promising alternative to the traditional PFGE method. Further studies are needed to accumulate more experience with genotyping using SNP analysis for the epidemiological investigation of outbreaks of C. perfringens. |
