A multi-omics approach reveals that lotus root polysaccharide iron ameliorates iron deficiency-induced testicular damage by activating PPARγ to promote steroid hormone synthesis.

Autor:	Dong PY; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China., Yuan S; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China., Chen Yan YM; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China., Chen Y; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China., Bai Y; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China., Dong Y; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China., Li YY; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China., Shen W; College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China., Zhang XF; College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266100, China. Electronic address: zhangxf106@qau.edu.cn.
Jazyk:	angličtina
Zdroj:	Journal of advanced research [J Adv Res] 2024 Sep 28. Date of Electronic Publication: 2024 Sep 28.
DOI:	10.1016/j.jare.2024.09.022
Abstrakt:	Iron deficiency is a common nutritional issue that seriously affects male reproductive health. Lotus root polysaccharide iron (LRPF), a novel nutritional supplement, may ameliorate the damage caused by iron deficiency, however, the mechanism is unclear. In this study, we comprehensively determined the benefits of LRPF on reproduction in iron-deficient mice by integrating transcriptomics, microbiomics and serum metabolomics. Microbiomics showed that LRPF could restore changes to the intestinal microbiota caused by iron deficiency. Metabolomics results showed that LRPF stabilised steroid hormone and fatty acid metabolism in iron-deficient mice, reduced the content of ethyl chrysanthemumate (EC) and ameliorated the reproductive impairment. The transcriptomic analysis showed that LRPF regulated steroid hormone synthesis and the peroxisome proliferator-activated receptor (PPAR) signalling pathway in iron-deficient mice. In vitro experiments showed that LRPF could promote steroid hormone synthesis in Leydig cells by activating PPARγ. In conclusion, this study highlights the advantage of LRPF to improve testicular development. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Production and hosting by Elsevier B.V.)
Databáze:	MEDLINE
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