Disruption of millipede-gut microbiota in E. pulchripes and G. connexa highlights the limited role of litter fermentation and the importance of litter-associated microbes for nutrition.

Autor: Nweze JE; Institute of Soil Biology and Biogeochemistry, Biology Centre CAS, České Budějovice, Czechia.; Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czechia., Gupta S; Institute of Soil Biology and Biogeochemistry, Biology Centre CAS, České Budějovice, Czechia.; Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czechia., Salcher MM; Institute of Hydrobiology, Biology Centre CAS, České Budějovice, Czechia., Šustr V; Institute of Soil Biology and Biogeochemistry, Biology Centre CAS, České Budějovice, Czechia., Horváthová T; Institute of Hydrobiology, Biology Centre CAS, České Budějovice, Czechia.; Department of Aquatic Ecology, EAWAG, Dübendorf, Switzerland., Angel R; Institute of Soil Biology and Biogeochemistry, Biology Centre CAS, České Budějovice, Czechia. roey.angel@bc.cas.cz.; Faculty of Science, University of South Bohemia in České Budějovice, České Budějovice, Czechia. roey.angel@bc.cas.cz.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Sep 28; Vol. 7 (1), pp. 1204. Date of Electronic Publication: 2024 Sep 28.
DOI: 10.1038/s42003-024-06821-2
Abstrakt: Millipedes are thought to depend on their gut microbiome for processing plant-litter-cellulose through fermentation, similar to many other arthropods. However, this hypothesis lacks sufficient evidence. To investigate this, we used inhibitors to disrupt the gut microbiota of juvenile Epibolus pulchripes (tropical, CH 4 -emitting) and Glomeris connexa (temperate, non-CH 4 -emitting) and isotopic labelling. Feeding the millipedes sterile or antibiotics-treated litter reduced faecal production and microbial load without major impacts on survival or weight. Bacterial diversity remained similar, with Bacteroidota dominant in E. pulchripes and Pseudomonadota in G. connexa. Sodium-2-bromoethanesulfonate treatment halted CH 4 emissions in E. pulchripes, but it resumed after returning to normal feeding. Employing 13 C-labeled leaf litter and RNA-SIP revealed a slow and gradual prokaryote labelling, indicating a significant density shift only by day 21. Surprisingly, labelling of the fungal biomass was somewhat quicker. Our findings suggest that fermentation by the gut microbiota is likely not essential for the millipede's nutrition.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje