Differential detection of SARS-CoV-2 variants and influenza A viruses utilizing a dual lateral flow strip based on colloidal gold-labeled monoclonal antibodies.

Autor: Li G; College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China., Wang X; College of Food and Drug, Luoyang Polytechnic, Luoyang 471023, China., Guo J; Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China., Wang Y; College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China., Liu X; Henan Medical College, Zhengzhou 451191, China., Wei Q; Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China., Zhang Y; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China., Sun Y; Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China., Fan L; Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China., Xing Y; Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China., Li Q; Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. Electronic address: 13838248151@163.com., Zhang G; College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China; Institute for Animal Health, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou University, Yangzhou 225009, China. Electronic address: zhanggaip@126.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 280 (Pt 4), pp. 136067. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1016/j.ijbiomac.2024.136067
Abstrakt: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the seasonal influenza virus are spreading among humans concurrently, especially with the ongoing replacement of mutant strains. It is challenging to differentiate between symptoms for therapy due to the comparable symptoms following infection with the SARS-CoV-2 variants and influenza viruses. Meanwhile, in order to achieve rapid point-of-care testing (POCT) to manage the spread of the disease, we developed a dual lateral flow strip based on colloidal gold-labeled monoclonal antibodies that can perform differential detection of SARS-CoV-2 variants and influenza A viruses (IAV) in this study. High-affinity monoclonal antibodies (mAbs) targeting SARS-CoV-2 and IAV were prepared to capture antigens and labeled with colloidal gold nanoparticles (AuNPs). Based on high-affinity mAbs, two targets were immobilized on one nitrocellulose (NC) membrane to establish the lateral flow strip (LFS) for differential diagnosis of SARS-CoV-2 and IAV. With no reactivity to other viruses, this LFS is extremely specific and can only identify SARS-CoV-2 and IAV. The LFS showed a limit of detection (LOD) of 4.88 ng/mL for the Omicron BA.2 RBD protein and 2.44 ng/mL for the nucleoprotein (NP) protein of H1N1. When analyzing 16 SARS-CoV-2 positive clinical samples, eight IAV positive clinical samples, and six negative samples that had already been pre-confirmed by commercial kits, its clinical application is effectively and accurately proven. These results demonstrated that the LFS integrated with AuNPs has great potential to facilitate quick, easy, and reliable POCT diagnosis for promoting the control of infectious diseases.
Competing Interests: Declaration of competing interest The authors declare no competing interests.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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