Bismuth-infused perivascular wrap facilitates delivery of mesenchymal stem cells and attenuation of neointimal hyperplasia in rat arteriovenous fistulas.

Autor: Barcena AJR; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; College of Medicine, University of the Philippines Manila, Manila 1000, Philippines., Perez JVD; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; College of Medicine, University of the Philippines Manila, Manila 1000, Philippines., Bernardino MR; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Damasco JA; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., San Valentin EMD; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Klusman C; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Baylor College of Medicine, Houston, TX 77030, USA., Martin B; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Baylor College of Medicine, Houston, TX 77030, USA., Canlas GM; Department of Chemistry, Lamar University, Beaumont, TX 77710, USA., Heralde FM 3rd; College of Medicine, University of the Philippines Manila, Manila 1000, Philippines., Fowlkes N; Department of Veterinary Medicine and Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Bouchard RR; Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Cheng J; Division of Nephrology, Department of Medicine, Selzman Institute for Kidney Health, Baylor College of Medicine, Houston, TX 77030, USA., Huang SY; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Melancon MP; Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; The University of Texas MD Anderson Cancer Center, UTHealth Houston, Graduate School of Biomedical Sciences, Houston, TX 77030, USA. Electronic address: mmelancon@mdanderson.org.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2025 Jan; Vol. 166, pp. 214052. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1016/j.bioadv.2024.214052
Abstrakt: Background: Mesenchymal stem cells (MSCs) have emerged as novel therapies for supporting arteriovenous fistula (AVF) maturation, and bioresorbable polymeric scaffolds have enabled sustained MSC delivery into maturing AVFs. However, the radiolucency of biopolymeric wraps prevents in vivo monitoring of their integrity and location, hindering long-term preclinical investigations.
Methods: We infused bismuth nanoparticles (BiNPs) into polycaprolactone (PCL) to fabricate an electrospun perivascular wrap capable of MSC delivery and conducive to longitudinal monitoring using conventional imaging. We tested the wraps' effects on the attenuation of markers of neointimal hyperplasia (i.e., endothelial dysfunction, hypoxia, and inflammation), the leading cause of AVF failure, in rats with induced chronic kidney disease (n = 3 per time point) for the following groups: control (no wrap), PCL wrap, PCL with MSCs, PCL-Bi (BiNP-infused wrap), and PCL-Bi with MSCs.
Results: Physicochemical characterization and in vitro biocompatibility tests revealed that BiNP infusion did not alter the wrap's non-cytotoxicity toward vascular cells, hemocompatibility, and capacity for MSC loading but facilitated long-term monitoring via micro-computed tomography. After 8 weeks, all treatment groups demonstrated significant improvement in wall-to-lumen ratio on ultrasonography (P < 0.001), neointima-to-lumen ratio on histomorphometry (P < 0.001), and attenuation of neointimal hypoxia on immunohistochemistry (P < 0.05). Compared to non-MSC wraps, MSC-loaded wraps not only attenuated endothelial dysfunction and neointimal inflammation but also reduced hypoxia and inflammation across all vascular layers.
Conclusion: These results demonstrate that MSC delivery through a radiopaque polymeric wrap could enhance AVF patency outcomes through the inhibition of multiple pathways inducing AVF failure.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: