Radiotracers for in situ infection imaging: Experimental considerations for in vitro microbial uptake of gallium-68-labeled siderophores.

Autor: Akter A; Institute of Pharmaceutical Science, Faculty of Life Science & Medicine, King's College London, London, UK., Cooper MS; School of Biomedical Engineering & Imaging Sciences, Faculty of Life Science & Medicine, King's College London, London, UK., Darwesh AMF; School of Biomedical Engineering & Imaging Sciences, Faculty of Life Science & Medicine, King's College London, London, UK; Department of Radiologic Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia., Hider RC; Institute of Pharmaceutical Science, Faculty of Life Science & Medicine, King's College London, London, UK., Blower PJ; School of Biomedical Engineering & Imaging Sciences, Faculty of Life Science & Medicine, King's College London, London, UK., Price NM; Directorate of Infection, Guy's & St Thomas' NHS Foundation Trust, London, UK., Lyons O; Department of Surgery, University of Otago, Christchurch, New Zealand., Schelenz S; Department of Microbiology, Kings College Hospital NHS Foundation Trust, London, UK., Mehra V; Department of Hematology, King's College Hospital NHS Foundation Trust, London, UK., Abbate V; Institute of Pharmaceutical Science, Faculty of Life Science & Medicine, King's College London, London, UK. Electronic address: vincenzo.abbate@kcl.ac.uk.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2024 Dec; Vol. 110 (4), pp. 116522. Date of Electronic Publication: 2024 Sep 07.
DOI: 10.1016/j.diagmicrobio.2024.116522
Abstrakt: In vitro screening of gallium-68( 68 Ga)-siderophores in pathogens relevant to infections is valuable for determining species specificity, their effect on cell viability, and potential clinical applications. As the recognition and internalization of siderophores relies on the presence of receptor- and/or siderophore-binding proteins, the level of uptake can vary between species. Here, we report in vitro uptake validation in Escherichia coli with its native siderophore, enterobactin (ENT) ([ 68 Ga]Ga-ENT), considering different experimental factors. Compared with other reporting methods of uptake, '% Added dose/10 9 CFU/mL (% AD/10 9 CFU/mL),' considering the total viable count, showed a better comparison among microbial species. Later, in vitro screening with [ 68 Ga]Ga-desferrioxamine B (DFO-B) showed high uptake by Staphylococcus aureus and S. epidermidis; moderate uptake by Pseudomonas aeruginosa; poor uptake by E. coli, Candida albicans, and Aspergillus fumigatus; and no uptake by Enterococcus faecalis and C. glabrata. Except for S. epidermidis, [ 68 Ga]Ga-DFO-B did not reduce the cell viability.
Competing Interests: Declaration of competing interest There are no competing interests from any authors.
(Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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