Ang-1 promotes tumorigenesis and mediates the anti-cancer effects of Artesunate on Choroidal melanoma via the regulation of Akt/mTOR signaling pathway.

Autor: Yao N; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Ma Q; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Yi W; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Liu Y; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Zhang Q; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Gao X; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Zhao X; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Wang H; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Lei K; Tumor Immunology and Cytotherapy of Medical Research Center and Key Laboratory of Pancreatic Disease Clinical Research (Shandong Province), The Affiliated Hospital of Qingdao University, Qingdao 266000, China., Sui A; Center Laboratory, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China., Luo W; Department of Ophthalmology, The Affiliated Hospital of Qingdao University, 266003, Qingdao, China. Electronic address: luowenjuan@qdu.edu.cn.
Jazyk: angličtina
Zdroj: Cytokine [Cytokine] 2024 Dec; Vol. 184, pp. 156771. Date of Electronic Publication: 2024 Sep 27.
DOI: 10.1016/j.cyto.2024.156771
Abstrakt: The impact of Ang-1 on tumors remains a subject of contention, with its mechanism of action exhibiting complexity in the progression of diverse tumor types. Ang-1 has been shown to promote the progression of glioma, glioma, esophageal and human cervical cancer, whereas it exerts inhibitory effects on the growth of breast and colon cancer. However, the specific function of Ang-1 in CM has not been clarified. This research aims to explore the function of Ang-1 on CM and the underlying mechanism. WB and qPCR were utilized to measure the expression levels of different factors in CM cells. Clonogenic, CCK-8 and Transwell migration assay were used to probe CM cells' proliferation and migration ability. Xenograft tumor model was used to testify the effect of Ang-1 and Artesunate (ART) on the growth of CM in vivo. We found Ang-1 promoted CM proliferation and migration, while it was inhibited by ART in vitro. Moreover, both ART treatment and Ang-1 knockdown had the effect of suppressing tumor growth in CM xenograft model. Mechanically, Ang-1 activated Akt/mTOR pathway and induced epithelial-mesenchymal transition (EMT) in CM cells. Furthermore, ART regulated Akt/mTOR pathway by decreasing the expression of Ang-1 in CM cells. Ang-1 promotes tumorigenesis of CM by regulating Akt/mTOR pathway, which can be inhibited by ART.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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