Antimicrobial resistance characterization of Enterococcus faecium, Enterococcus faecalis and Enterococcus hirae isolated from marine coastal recreational waters in the State of São Paulo, Brazil.

Autor:	Santiago GS; School of Public Health, Environmental Health Department, University of São Paulo, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil., Dropa M; School of Public Health, Environmental Health Department, University of São Paulo, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil., Martone-Rocha S; School of Public Health, Environmental Health Department, University of São Paulo, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil., Dos Santos TP; School of Public Health, Environmental Health Department, University of São Paulo, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil., de Moura Gomes VT; CETESB - Environmental Company of São Paulo State, Av. Professor Frederico Hermann Jr, 345, São Paulo, SP 05459-900, Brazil., Barbosa MRF; CETESB - Environmental Company of São Paulo State, Av. Professor Frederico Hermann Jr, 345, São Paulo, SP 05459-900, Brazil; Environmental Health Department, NARA - Center for Research in Environmental Risk Assessment, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil., Razzolini MTP; School of Public Health, Environmental Health Department, University of São Paulo, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil; Environmental Health Department, NARA - Center for Research in Environmental Risk Assessment, Av. Dr Arnaldo 715, São Paulo, SP 01246-904, Brazil E-mail: razzolini@usp.br.
Jazyk:	angličtina
Zdroj:	Journal of water and health [J Water Health] 2024 Sep; Vol. 22 (9), pp. 1628-1640. Date of Electronic Publication: 2024 Aug 23.
DOI:	10.2166/wh.2024.098
Abstrakt:	Coastal water quality is facing increasing threats due to human activities. Their contamination by sewage discharges poses significant risks to the environment and public health. We aimed to investigate the presence of antibiotic-resistant Enterococcus in beach waters. Over a 10-month period, samples were collected from four beaches in the State of São Paulo (Brazil). Enterococcus isolates underwent matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) and molecular analysis for accurate genus and species identification. The antimicrobial susceptibility for 14 antibiotics was evaluated using the disc diffusion method followed by a multidrug-resistance (MDR) classification. PCR amplification method was used to detect antimicrobial resistance genes (ARGs). Our findings revealed the prevalence of Enterococcus faecalis , E. faecium and E. hirae . Out of 130 isolates, 118 were resistant to multiple antibiotics. The detection of resistance genes provided evidence of the potential transfer of antibiotic resistance within the environment. Our findings underscore the necessity for continuous research and surveillance to enhance understanding of the pathogenicity and antimicrobial resistance mechanisms of Enterococcus , which is crucial to implement effective measures to preserve the integrity of coastal ecosystems. Competing Interests: The authors declare there is no conflict. (© 2024 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY-NC-ND 4.0), which permits copying and redistribution for non-commercial purposes with no derivatives, provided the original work is properly cited (http://creativecommons.org/licenses/by-nc-nd/4.0/).)
Databáze:	MEDLINE
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