| Autor: |
Ardali R; Institute of Virology and Immunology, 3147 Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland., Garcia-Nicolas O; Institute of Virology and Immunology, 3147 Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland., Ollagnier C; Swine Research Unit, Agroscope, 1725 Posieux, Switzerland., Sánchez Carvajal JM; Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine, University of Córdoba, 14014 Córdoba, Spain., Levy M; Swine Research Unit, Agroscope, 1725 Posieux, Switzerland., Yvernault P; Swine Research Unit, Agroscope, 1725 Posieux, Switzerland., de Aboim Borges Fialho de Brito F; Institute of Virology and Immunology, 3147 Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland., Summerfield A; Institute of Virology and Immunology, 3147 Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland. |
| Abstrakt: |
The non-specific protective effects offered by the concept of "innate immune memory" might represent a promising strategy to tackle early-life threatening infections. Here we tested the potential of an in vitro selected β-glucan in inducing trained immunity using an in vivo porcine model. We assessed the leukocyte transcriptome using blood transcriptomic module (BTM), proinflammatory cytokines, and clinical scoring after a first "training" and a second "stimulation" phase. The possible induction of innate immune memory was tested during a "stimulation" by an LPS-adjuvanted Mycoplasma hyopneumoniae vaccine (Hyogen ® ) one day after weaning. Following the "training", no major group differences were found, with the exception of a plasma TNF that was only induced by Adj and Adj_BG treatment. After vaccination, all groups developed similar antibody responses. A significant induction of plasma TNF and IL-1β was found in groups that received Adj and Adj_BG. However, following vaccination, the expected early innate BTMs were only induced by the PBS group. In conclusion, the adjuvant alone, adjuvant-formulated β-glucan, or orally applied β-glucan were unable to enhance innate immune reactivity but rather appeared to promote innate immune tolerance. Such an immune status could have both positive and negative implications during this phase of the piglet's life. |
