Characterization of the Monkeypox Virus [MPX]-Specific Immune Response in MPX-Cured Individuals Using Whole Blood to Monitor Memory Response.

Autor:	Petruccioli E; Translational Research Unit, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Sbarra S; Translational Research Unit, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Vita S; Highly Infectious Diseases Isolation Unit, Clinical Department, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Salmi A; Translational Research Unit, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Cuzzi G; Translational Research Unit, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., De Marco P; Highly Infectious Diseases Isolation Unit, Clinical Department, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Matusali G; Laboratory of Virology and Biosafety Laboratories, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Navarra A; Clinical Epidemiology Unit, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Pierelli L; Unità Operativa Complessa (UOC) Transfusion Medicine and Stem Cell, San Camillo Forlanini Hospital, 00149 Rome, Italy., Grifoni A; Center for Vaccine Innovation, La Jolla Institute for Immunology, La Jolla, CA 92037, USA., Sette A; Center for Vaccine Innovation, La Jolla Institute for Immunology, La Jolla, CA 92037, USA.; Department of Medicine, Division of Infectious Diseases and Global Public Health, University of California, San Diego (UCSD), La Jolla, CA 92037, USA., Maggi F; Laboratory of Virology and Biosafety Laboratories, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Nicastri E; Highly Infectious Diseases Isolation Unit, Clinical Department, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy., Goletti D; Translational Research Unit, National Institute for Infectious Diseases 'Lazzaro Spallanzani' IRCCS, 00149 Rome, Italy.
Jazyk:	angličtina
Zdroj:	Vaccines [Vaccines (Basel)] 2024 Aug 26; Vol. 12 (9). Date of Electronic Publication: 2024 Aug 26.
DOI:	10.3390/vaccines12090964
Abstrakt:	Background: Monkeypox (Mpox) is a zoonotic disease caused by monkeypox virus (MPXV), an Orthopoxvirus (OPXV). Since we are observing the first MPXV outbreak outside the African continent, the general population probably does not have a pre-existing memory response for MPXV but may have immunity against the previous smallpox vaccine based on a live replicating Vaccinia strain (VACV). Using a whole blood platform, we aim to study the MPXV- T-cell-specific response in Mpox-cured subjects. Methods: We enrolled 16 subjects diagnosed with Mpox in the previous 3-7 months and 15 healthy donors (HD) with no recent vaccination history. Whole blood was stimulated overnight with MPXV and VACV peptides to elicit CD4 and CD8 T-cell-specific responses, which were evaluated by ELISA and multiplex assay. Results: Mpox-cured subjects showed a significant IFN-γ T-cell response to MPXV and VACV. Besides IFN-γ, IL-6, IP-10, IL-8, IL-2, G-CSF, MCP-1, MIP1-α, MIP-1β, IL-1Rα, and IL-5 were significantly induced after specific stimulation compared to the unstimulated control. The specific response was mainly induced by the CD4 peptides MPX-CD4-E and VACV-CD4. Conclusions: We showed that MPXV-specific responses have a mixed Th1- and Th2-response in a whole blood platform assay, which may be useful for monitoring the specific immunity induced by vaccination or infection.
Databáze:	MEDLINE
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