| Autor: |
Gambichler T; Department of Dermatology, Ruhr-University Bochum, 44791 Bochum, Germany.; Department of Dermatology, Dortmund Hospital, Faculty of Health, School of Medicine, University Witten/Herdecke, 44137 Dortmund, Germany.; Department of Dermatology, Christian Hospital Unna, 59423 Unna, Germany., Goesmann S; Department of Dermatology, Ruhr-University Bochum, 44791 Bochum, Germany., Skrygan M; Department of Dermatology, Ruhr-University Bochum, 44791 Bochum, Germany., Susok L; Department of Dermatology, Ruhr-University Bochum, 44791 Bochum, Germany.; Department of Dermatology, Dortmund Hospital, Faculty of Health, School of Medicine, University Witten/Herdecke, 44137 Dortmund, Germany., Schütte C; Department of Internal Medicine, Ruhr-University Bochum, 44791 Bochum, Germany., Hamdani N; Department of Molecular and Experimental Cardiology, Ruhr-University Bochum, 44791 Bochum, Germany.; Department of Cardiology, Ruhr-University Bochum, 44791 Bochum, Germany.; Institute of Physiology, Ruhr-University Bochum, 44801 Bochum, Germany., Schmidt W; Department of Internal Medicine, Ruhr-University Bochum, 44791 Bochum, Germany. |
| Abstrakt: |
Immune responses of the epithelia of the upper respiratory tract are likely crucial in early inhibition of the viral replication and finally clearance of SARS-CoV-2. We aimed to compare the expression profiles of antimicrobial peptides/proteins (AMPs) and related cytokines observed in the nasopharynx of SARS-CoV-2-infected patients and non-infected controls and to assess the associations between these parameters and COVID-19 patients' outcomes. We included 45 subjects who had tested positive for SARS-CoV-2 and 22 control subjects who had tested negative for SARS-CoV-2. Biomaterial for SARS-CoV-2 detection, as well as gene and protein expression studies, was obtained from all subjects using nasopharyngeal swabs which were performed a maximum of 7 days before inclusion in the study. Univariable and multivariable statistics were performed. When compared to the controls, the mRNA expression levels of human β-defensin 1 (hBD-1), LL-37, and trappin-2 were significantly higher in specimens of nasopharyngeal swabs from COVID-19 patients. Protein expression of hBD-1 was also increased in the COVID-19 group. mRNA expression levels of interferon-ɣ (IFN-ɣ), tumor necrosis factor- ɑ (TNF-ɑ), and interleukin-6 (IL-6) measured in SARS-CoV-2-infected patients were significantly higher than those observed in the controls, which could also be confirmed in the protein levels of IFN-ɣ and IL-6. A significant correlation between mRNA and protein levels could be observed only for IL-6. Univariable analysis revealed that low IFN-ɣ mRNA levels were associated with severe/fatal outcomes. The occurrence of COVID-19 pneumonia was significantly associated with lower expression levels of IL-6 mRNA, IFN-ɣ mRNA, and TNF-ɑ mRNA. Concerning the severe/fatal outcomes, the multivariable logistic regression model revealed that none of the aforementioned parameters remained significant in the model. However, the logistic regression model revealed that higher TNF-ɑ mRNA expression was a significant independent predictor of absence of pneumonia [odds ratio: 0.35 (95% CI 0.14 to 0.88, p = 0.024)]. In conclusion, nasopharyngeal expression of AMPs (hBD-1, LL-37, and trappin-2) and cytokines (IL-6, IFN-ɣ, and TNF-ɑ) is upregulated in response to early SARS-CoV-2 infection, indicating that these AMPs and cytokines play a role in the local host defense against the virus. Upregulated nasopharyngeal TNF-ɑ mRNA expression during the early phase of SARS-CoV-2 infection was a significant independent predictor of the absence of COVID-19 pneumonia. Hence, high TNF-ɑ mRNA expression in the nasopharynx appears to be a protective factor for lung complications in COVID-19 patients. |
